PXD056399

PXD056399 is an original dataset announced via ProteomeXchange.

Title	Structural variation of types IV-A1- and IV-A3-mediated CRISPR interference
Description	CRISPR-Cas mediated DNA-interference typically relies on sequence-specific binding and nucleolytic degradation of foreign genetic material. Type IV-A CRISPR-Cas systems diverge from this general mechanism, using a nuclease-independent interference pathway to suppress gene expression for gene regulation and plasmid competition. To understand how the type IV-A system associated effector complex achieves this interference, we determined cryo-EM structures of two evolutionarily distinct type IV-A complexes (types IV-38 A1 and IV-A3) bound to cognate DNA-targets in the presence and absence of the type IV-A signature DinG effector helicase. The structures reveal how the effector complexes recognize the protospacer adjacent motif and target-strand DNA to form an R-loop structure. Additionally, we reveal differences between types IV-A1 and IV-A3 in DNA interactions and structural motifs that allow for in trans recruitment of DinG. Our study provides a detailed view of type IV-A mediated DNA-interference and presents a structural foundation for engineering type IV-A-based genome editing tools.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_04:57:12.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rimvydė Čepaitė
SpeciesList	scientific name: Pseudomonas oleovorans; NCBI TaxID: 301;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-30 09:27:02	ID requested
⏵ 1	2024-10-08 04:57:12	announced

Dataset with its publication pending

submitter keyword: cryo-EM,type IV CRISPR-Cas

Patrick Pausch
contact affiliation	LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Saulėtekio al. 7, 10257 Vilnius, Lithuania
contact email	patrick.pausch@gmc.vu.lt
lab head
Rimvydė Čepaitė
contact affiliation	LSC-EMBL Partnership Institute for Genome Editing Technologies, Life Sciences Center, Vilnius University, Saulėtekio al. 7, 10257 Vilnius, Lithuania
contact email	rimvyde.cepaite@gmc.vu.lt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD056399

PRIDE project URI

• PRIDE
  1. PXD056399
     1. Label: PRIDE project
     2. Name: Structural variation of types IV-A1- and IV-A3-mediated CRISPR interference