PXD056186

PXD056186 is an original dataset announced via ProteomeXchange.

Title	An eRNA Transcription Checkpoint For Diverse Signal-dependent Enhancer Activation Programs
Description	The evidence that several signal and ligand-dependent pathways function by activating regulatory enhancer programs suggests that a “checkpoint” strategy may underline activation of some or even many diversely-regulated enhancers. Here, we report a molecular mechanism common to several acute signal- and ligand-dependent enhancer activation programs based on release of a shared eRNA transcription checkpoint. This requires recruitment of a DNA-PKcs-phosphorylated RING finger repressor, KAP1, functioning as a modulator, inhibiting its association with 7SK and E3 SUMO ligase activity on the CDK9 subunit of P-TEFb, facilitating formation of an activated P-TEFb complex, licensing eRNA elongation. Overcoming this checkpoint for signal-dependent enhancer activation occurs in diverse pathways including estrogen receptor α, NF-κB-regulated proinflammatory, androgen receptor and neuronal depolarization. Therefore, a specific strategy required to convert a basal state enhancer P-TEFb complex to an active state to release a conserved checkpoint is apparently employed by several functionally-important signal/ligand-regulated regulatory enhancers to implement the instructions of endocrine/paracrine system
HostingRepository	PRIDE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-21_08:21:03.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Yuan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 10:24:40	ID requested
⏵ 1	2025-02-21 08:21:03	announced

Dataset with its publication pending

submitter keyword: IP-MS

Michael G Rosenfeld
contact affiliation	University of California, San Diego
contact email	mrosenfeld@ucsd.edu
lab head
Wei Yuan
contact affiliation	University of California, San Diego
contact email	w3yuan@health.ucsd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD056186

PRIDE project URI

• PRIDE
  1. PXD056186
     1. Label: PRIDE project
     2. Name: An eRNA Transcription Checkpoint For Diverse Signal-dependent Enhancer Activation Programs