PXD056184

PXD056184 is an original dataset announced via ProteomeXchange.

Title	Validation of potential growth-related proteins in chick vitreous during emmetropization using targeted-based proteomics (MRMHR)
Description	The relatively new high-resolution MRM (MRMHR) approach with the Triple-TOF MS running under nanoflow was adopted in this study to solidify further the findings of SWATH-MS results of differentially expressed proteins found across the time points in the chick vitreous for the benefit of low peptide amount consumption. Twelve up-regulated and 26 down-regulated proteins were found across all time points compared to day 7 using SWATH-MS. Several DEPs, such as alpha-fetoprotein, cadherins family group, neurocan, and reelin involved in structural and growth-related pathways, were validated for the first time using MRMHR under this experimental condition.
HostingRepository	PRIDE
AnnounceDate	2024-09-25
AnnouncementXML	Submission_2024-09-25_08:04:38.974.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jimmy Ka-wai Cheung
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	iodoacetic acid derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-24 09:28:51	ID requested
⏵ 1	2024-09-25 08:04:39	announced

Dataset with its publication pending

submitter keyword: Vitreous, SWATH-MS, MRMHR

Thomas Chuen Lam
contact affiliation	1. Centre for Myopia Research, School of Optometry, The Hong Kong Polytechnic University, Hong Kong 2. Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong 3. Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, Hong Kong
contact email	thomas.c.lam@polyu.edu.hk
lab head
Jimmy Ka-wai Cheung
contact affiliation	Center for eye and vision research, LTD, HK The Hong Kong Polytechinic univerisity, HK
contact email	kawai.cheung@cevr.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD056184

PRIDE project URI

• PRIDE
  1. PXD056184
     1. Label: PRIDE project
     2. Name: Validation of potential growth-related proteins in chick vitreous during emmetropization using targeted-based proteomics (MRMHR)