PXD055965

PXD055965 is an original dataset announced via ProteomeXchange.

Title	Multi tissue proteomic analysis of cold exposure
Description	This study explored the proteomic basis of cold adaptation, quantifying 11,394 proteins across 6 metabolic tissues and plasma. Systems level analysis revealed an enrichment for proteins involved in metabolism. In brown adipose tissue (BAT), cold adaptation was associated with enhanced glucose utilisation, including select remodelling of upper glycolysis to enhance superoxide production and UCP1 activity, and a downregulation of proteins involved in fatty acid oxidation. Strikingly, opposite changes were observed in liver and white adipose tissues, including upregulation of proteins involved in gluconeogenesis and lipolysis, respectively. This suggests that coordinated changes in many organs are required for maximal BAT thermogenesis. This coordination also associated with cold-regulated alterations to the plasma proteome, including elevated Fetuin A that is consistent with enhanced lipolysis in white adipose. Finally, overlapping but inverse changes in cold-exposed and diet-induced obese mice support impaired BAT activity as a driver of obesity.
HostingRepository	PRIDE
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-22_19:09:24.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David James
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-18 00:39:06	ID requested
⏵ 1	2025-04-22 19:09:24	announced

Dataset with its publication pending

submitter keyword: white adipose,Mouse, brown adipose, liver, muscle

David Ernest James
contact affiliation	Charles Perkins Centre, The University of Sydney
contact email	david.james@sydney.edu.au
lab head
David James
contact affiliation	The University of Sydney
contact email	david.james@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD055965

PRIDE project URI

• PRIDE
  1. PXD055965
     1. Label: PRIDE project
     2. Name: Multi tissue proteomic analysis of cold exposure