PXD055865

PXD055865 is an original dataset announced via ProteomeXchange.

Title	Glycosite Mapping and in situ Mass Spectrometry Imaging of MUC2 Glycopeptides via On-slide Mucinase Digestion
Description	Aberrant mucin expression and glycosylation are hallmarks of cancer, but how these changes promote malignant processes are not well understood. Solid tumors are highly heterogeneous in their cellular and molecular composition, and many advanced spatial techniques have emerged in recent years to study the tumor microenvironment (TME) for better understanding disease progression. Spatially resolved glycoprotein analyses typically detect either the protein or glycan components, but not both. We developed a workflow using a dual mass spectrometry approach to map the location of intact glycopeptides in mucinous tumors, enabled by on-tissue digestion with the mucin-specific protease StcE. Future applications of this method on larger patient cohorts will enhance our understanding of glycans in malignancy, identify disease biomarkers, and define therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_22:29:06.920.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Lowery
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	O-glycosylated residue; complex glycosylation
Instrument	Orbitrap Eclipse; timsTOF fleX; Bruker Daltonics solarix series; timsTOF fleX MALDI-2

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-14 10:20:42	ID requested
⏵ 1	2026-04-05 22:29:07	announced

Dataset with its publication pending

submitter keyword: None

Stacy Malaker
contact affiliation	Department of Chemistry, Yale University, New Haven, CT 06520
contact email	stacy.malaker@yale.edu
lab head
Sarah Lowery
contact affiliation	Yale University
contact email	sarah.lowery@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD055865

PRIDE project URI

• PRIDE
  1. PXD055865
     1. Label: PRIDE project
     2. Name: Glycosite Mapping and in situ Mass Spectrometry Imaging of MUC2 Glycopeptides via On-slide Mucinase Digestion