PXD055804

PXD055804 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of Arabidopsis Lon1 protease mutant
Description	Intracellular selective proteolysis is an important protein quality control process for maintenance of protein homeostasis by removing either the defective polypeptides or deleterious protein aggregates. The ATP-dependent Lon protease is a key component of protein quality control and highly conserved across the kingdoms of living organisms. In Arabidopsis thaliana Lon1 (At5g26860) is dual targeted to mitochondria and chloroplasts. Lon1 mutant plants (lon1-1) exhibit a post-embryonic growth retardation phenotype accompanied with aberrant mitochondria morphology and reduced respiratory capacity. To identify the effect of loss of Lon1 proteolytic activity on the protein level of the plant, the proteome profile of lon1-1 mutant was analyzed by mass spectrometry and compared to the proteome of the wild type plants (Col-0) used as a reference. Total protein extraction was performed under denature conditions of 8 days old seedling, grown vertically on petri dishes at 22 o C in the dark from independent biological replicates. The protein extract was subjected to Ultra High Pressure nanoLC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2025-06-10
AnnouncementXML	Submission_2025-06-10_05:18:00.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-11 23:12:06	ID requested
⏵ 1	2025-06-10 05:18:01	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, protein homeostasis, mutant, protease, protein quality control,Lon

Stamatis Rigas
contact affiliation	Department of Biotechnology, Agricultural University of Athens, Iera Odos 75, Athens 118 55, Greece
contact email	srigas@aua.gr
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD055804
     1. Label: PRIDE project
     2. Name: Proteome analysis of Arabidopsis Lon1 protease mutant