PXD055750

PXD055750 is an original dataset announced via ProteomeXchange.

Title	A proteome-wide dependency map of protein interaction motifs
Description	Short linear motifs (SLiMs) are the most ubiquitous protein interaction modules in the unstructured regions of the human proteome. Despite their central role in protein function, our understanding of the contribution of SLiMs to cellular homeostasis remains limited. To address this, we designed base editor libraries to precisely mutate all curated SLiMs and a set of computationally predicted instances defined by SLiM-like evolutionary patterns. By targeting 7,293 SLiM containing regions with 80,473 mutations, we define an amino acid resolution SLiM dependency map identifying 450 known and 264 predicted SLiMs required for normal cell proliferation. Notably, the vast majority of essential predicted SLiMs belong to novel classes of SLiMs. We also uncover the functions of several predicted SLiMs in core biological processes and provide mechanistic insight into disease causing mutations. Collectively, our study provides a proteome-wide publicly accessible resource on SLiM essentiality and highlights the presence of numerous uncharacterised essential SLiMs in the human proteome.
HostingRepository	PRIDE
AnnounceDate	2026-01-14
AnnouncementXML	Submission_2026-01-14_14:52:26.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-09-09 10:23:12	ID requested
⏵ 1	2026-01-14 14:52:27	announced

Dataset with its publication pending

submitter keyword: cellular homeostasis,Short linear motifs,proteome

Matthias Mann
contact affiliation	Max Planck Institute of Biochemistry, Germany Novo Nordisk Foundation Center for Protein Research,Denmark
contact email	mmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD055750

PRIDE project URI

• PRIDE
  1. PXD055750
     1. Label: PRIDE project
     2. Name: A proteome-wide dependency map of protein interaction motifs