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PXD055592

PXD055592 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleParallel genome-scale CRISPR-Cas9 screens uncouple human pluripotent stem cell identity versus fitness
DescriptionPluripotent stem cells are defined by their self-renewal capacity, which is the ability of the stem cells to proliferate indefinitely while maintaining the pluripotent identity essential for their ability to differentiate into any somatic cell lineage. However, understanding the mechanisms that control stem cell fitness versus the pluripotent cell identity is challenging. To investigate the interplay between these two aspects of pluripotency, we performed four parallel genome-scale CRISPR-Cas9 loss-of-function screens interrogating stem cell fitness in hPSC self-renewal conditions, and the dissolution of the primed pluripotency identity during early differentiation. Comparative analyses led to the discovery of genes with distinct roles in pluripotency regulation, including mitochondrial and metabolism regulators crucial for stem cell fitness, and chromatin regulators that control pluripotent identity during early differentiation. We further discovered a core set of factors that control both stem cell fitness and pluripotent identity, including a network of chromatin factors that safeguard pluripotency. Our unbiased and systematic screening and comparative analyses disentangle two interconnected aspects of pluripotency, provide rich datasets for exploring pluripotent cell identity versus cell fitness, and offer a valuable model for categorizing gene function in broad biological contexts.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:57:42.822.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSimone Sidoli
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-05 09:56:21ID requested
12024-09-05 10:29:16announced
22024-10-22 06:57:43announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human embryonic stem cells (hESCs), pluripotency, functional genomics, human pluripotent stem cells (hPSCs), cell fitness, genome-scale CRISPR-Cas9 genetic screening, differentiation, OTUD5, self-renewal
Contact List
Simone Sidoli
contact affiliationDepartment of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, United States
contact emailsimone.sidoli@einsteinmed.edu
lab head
Simone Sidoli
contact affiliationAlbert Einstein College of Medicine
contact emailsimone.sidoli@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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