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PXD055498

PXD055498 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3
DescriptionThe subcortical maternal complex (SCMC) is essential for the maintenance of female fertility in mammals. Most pathogenic mutations in SCMC-encoding genes result in female reproductive failure characterized by early embryonic arrest. Assembled in oocytes, the SCMC maintains the normal cleavage of early embryos, but the mechanism regulating this function remains unclear. Here, we report that 14-3-3, a multifunctional protein, is a component of the SCMC. By resolving the cryoelectron microscopy structure of the 14-3-3-containing SCMC, we revealed that specific phosphorylation sites in TLE6 contribute to the recruitment of 14-3-3, which is indispensable for the role of the SCMC in maintaining the cleavage of mouse early embryos. Mechanistically, during the maternal-to-embryo transition, the SCMC stabilizes the 14-3-3 protein, and an acute reduction in 14-3-3 protein expression in zygotes impairs their further development to blastocysts. The SCMC containing 14-3-3 contributes to the proper control of CDC25B, thus ensuring the activation of the maturation-promoting factor and mitotic entry in mouse zygotes. Notably, we proved that the recruitment of 14-3-3 to the SCMC and the molecular link between the SCMC and CDC25B are conserved in human oocytes/embryos. This study reveals the molecular mechanism through which the SCMC regulates the cell cycle in early embryos and represents a breakthrough in fully elucidating the functions of the SCMC in mammalian early embryogenesis.
HostingRepositoryiProX
AnnounceDate2024-09-03
AnnouncementXMLSubmission_2024-09-03_00:23:35.108.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZihan Zhang
SpeciesList scientific name: Spodoptera frugiperda; NCBI TaxID: 7108; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-09-03 00:20:31ID requested
12024-09-03 00:23:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: 14-3-3 protein, SCMC, maternal effect gene.
Contact List
Dong Deng
contact affiliationSichuan University
contact emaildengd@scu.edu.cn
lab head
Zihan Zhang
contact affiliationSichuan University
contact emailm18710996428@163.com
dataset submitter
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