⮝ Full datasets listing
PXD054138
PXD054138 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator dependent tRNA modifications |
| Description | Casein kinase 1 (CK1) family members are crucial for ER-Golgi trafficking, calcium signaling, DNA repair, tRNA modifications and circadian rhythmicity. Whether and how substrate interactions and kinase autophosphorylation contribute to these diverse cellular processes and CK1 plasticity remains largely unknown. Here, we undertake a comprehensive phylogenetic, cellular and molecular characterization of Hrr25 from budding yeast and identify isozyme CK1 epsilon as its human ortholog. We analyze the effect of Hrr25 depletion and catalytically inactive mutants in vivo and show that perturbations in CK1 activity lead to pleiotropic stress-induced growth defects, morphological abnormalities and loss of Elongator-dependent tRNA modification. We use purified Hrr25 protein to identify distinct autophosphorylation patterns and phospho-sites on several physiological substrates in vitro and find that only human isozyme CK1epsilon can replace yeast Hrr25 functions essential for tRNA modification and cell proliferation in vivo. Furthermore, we demonstrate that human and yeast CK1 orthologs share conserved autophosphorylation sites, which regulate their activities and mutually exclusive interactions with Sit4, a phosphatase antagonist of Hrr25, and Elongator subunit Elp1. Thus, autophosphorylation controls CK1 activity and regulates the tRNA modification pathway. Our data offer mechanistic insights into regulatory roles of CK1 that are conserved from yeast to human cells and reveal a complex phosphorylation network behind CK1 plasticity. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-26 |
| AnnouncementXML | Submission_2025-11-26_03:18:22.138.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Urszula Jankowska |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | Phospho; Oxidation; Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-07-23 04:02:44 | ID requested | |
| ⏵ 1 | 2025-11-26 03:18:23 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: yeast Hrr25, kinase 39 autophosphorylation, Elongator complex, Casein kinase 1 epsilon |
Contact List
| Sebastian Glatt | |
|---|---|
| contact affiliation | Malopolska Centre of Biotechnology (MCB), Jagiellonian University |
| contact email | sebastian.glatt@uj.edu.pl |
| lab head | |
| Raffael Schaffrath | |
| contact affiliation | Institute of Biology, Department of Microbiology, University of Kassel |
| contact email | schaffrath@uni-kassel.de |
| lab head | |
| Urszula Jankowska | |
| contact affiliation | Jagiellonian University |
| contact email | urszula.jankowska@uj.edu.pl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095414/ |
