PXD054112

PXD054112 is an original dataset announced via ProteomeXchange.

Title	Single-Cell Secretome Profiling by NanoLC-MS/MS Analysis
Description	Here, we reported a novel single cell secretome profiling method that integrates cell metabolic labeling, on-capillary enrichment based on click chemistry, in-situ sample preparation, and nanoLC-MS/MS analysis. By this method, an average of circa 389 protein groups were confidently identified as the secretome from single HeLa cells, an order of magnitude higher than previously reported targeted secretome analysis by antibody recognition.
HostingRepository	jPOST
AnnounceDate	2025-11-07
AnnouncementXML	Submission_2025-11-07_07:00:13.835.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingyun He
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-22 06:02:54	ID requested
⏵ 1	2025-11-07 07:00:14	announced

Dataset with its publication pending

submitter keyword: single-cell, secretome, LC-MS, click chemistry

Lihua Zhang
lab head
Yingyun He
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Science
dataset submitter

jPOST dataset URI

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