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PXD054003

PXD054003 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe hGID GID4 E3 ubiquitin ligase complex targets ARHGAP11A to regulate cell migration
DescriptionThe human GID (hGID) complex is a key E3 ligase regulating multiple cellular processes, including cell cycle progression and metabolic activity. However, the biological functions controlled by hGID remain poorly investigated. Here, we show that the hGID substrate receptor GID4 regulates cell migration and growth. Biochemical and cellular assays combined with proximity-dependent biotinylation (BioID2) uncovered that GID4 targets ARHGAP11A for degradation. Knockdown of GID4 or inhibition of the GID4 substrate binding pocket impairs motility and directed cell movement, whereas abrogation of ARHGAP11A significantly rescues this cell migration defect. GID4 inhibits cell migration by degrading ARHGAP11A thereby preventing its accumulation at the cell periphery where it turns off RhoA activity. Moreover, our BioID2-GID4 screens revealed a variety of substrate profiles beyond Pro/N-degron motifs with functions in microtubule binding, cytoskeletal dynamics and other biological processes. Overall, we identified cellular functions and a wide range of physiological hGID degradation substrates, which paves the way for deciphering molecular pathways regulated by hGID E3 ligase activity through its GID4 substrate receptor.
HostingRepositoryPRIDE
AnnounceDate2024-09-24
AnnouncementXMLSubmission_2024-09-24_04:29:39.187.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHalil Bagci
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-17 03:22:30ID requested
12024-09-24 04:29:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: E3 Ligase, RhoA, ARHGAP11A,GID, Cell migration, Ubiquitin
Contact List
Matthias Peter
contact affiliationInstitute of Biochemistry (IBC), ETH Zurich, Switzerland
contact emailmatthias.peter@bc.biol.ethz.ch
lab head
Halil Bagci
contact affiliationETH Zurich
contact emailhalil.bagci@mail.mcgill.ca
dataset submitter
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Dataset FTP location
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