PXD053637
PXD053637 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Cysteine redoxome landscape in mouse brown adipose tissue under acute cold exposure |
| Description | Reversible cysteine post-translational modifications serve as a "switch" for protein structure-function dynamics. While reversible cysteine oxidation in uncoupling protein 1 is known to play a role in brown fat thermogenesis, the full cysteine redoxome affected by cold exposure remains unexplored. We established a strategy for comprehensively mapping the cysteine redoxome by pinpointing oxidized and reduced cysteine residues in the brown adipose tissue of mice under room temperature and acute cold exposure. We identified over 8000 labeled cysteine residues under room and cold temperatures. Cold exposure shifted the cysteine redox states toward oxidation. Cold-sensitive reactive cysteine residues were enriched in biological processes and molecular functions associated with thermogenesis pathways. The presence of proximal positively charged and negatively charged amino acids determined the highly reactive and non-reactive cysteine residues, respectively, under cold exposure. Our findings broaden the landscape of cold-sensitive proteome and identify potential therapeutic targets to fine-tune thermogenesis. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-01-31 |
| AnnouncementXML | Submission_2025-01-31_02:06:29.682.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Takumi Nishiuchi |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | L-methionine sulfoxide; unknown modification; unknown modification; unknown modification; alpha-amino acetylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-07-04 01:48:29 | ID requested | |
| ⏵ 1 | 2025-01-31 02:06:30 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: redoxome, mouse, LFQ, DDA |
Contact List
| Toshinari Takamura | |
|---|---|
| lab head | |
| Takumi Nishiuchi | |
| contact affiliation | Kanazawa University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003198/ |
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