PXD053428

PXD053428 is an original dataset announced via ProteomeXchange.

Title	Conserved role of the SERK‒BIR module in development and immunity across land plants
Description	In the model angiosperm Arabidopsis thaliana, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASES (SERKs), which are subfamily II of leucine-rich repeat receptor-like kinases (LRR-RLKs), play diverse roles in development and immunity as co-receptors for a multitude of LRR-RLKs including BRASSINOSTEROID INSENSITIVE 1 (BRI1) and FLAGELLIN SENSITIVE 2 (FLS2). The conserved tyrosine (Y) residue in AtSERK3 plays a crucial role in signaling specificity for differentiating AtBRI1- and AtFLS2-mediated pathways. BRI ASSOCIATED RECEPTOR KINASE 1 (BAK1)-INTERACTING RECEPTOR-LIKE KINASES (BIRs) interact with SERKs under resting conditions, negatively regulating SERK-mediated pathways. SERK and BIR are highly conserved in land plants, whereas BRI1 and FLS2 homologs are absent or poorly conserved in bryophyte lineages. Very little is known about biological functions of SERK homologs in non-flowering plants. The genome of the model liverwort Marchantia polymorpha encodes the single SERK and BIR homolog, namely MpSERK and MpBIR. We here show that Mpserk disruptants display growth and developmental defects with no observable sexual and vegetative reproduction. Complementation analysis revealed a contribution of the conserved Y residue of MpSERK in growth. Proximity labelling-based interactomics of MpSERK identified MpBIR as an interactor. Mpbir disruptants displayed defects in reproductive organ development. Transcriptomes of Mpserk and Mpbir showed antagonistic patterns, suggesting MpBIR functions as a MpSERK repressor. We revealed that the pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 could hardly grow on Mpbir, indicating a significant role of the MpSERK‒MpBIR module in immunity. Taken together, we propose that the SERK-BIR functional module was already established in the last common ancestor of land plants for regulating both development and immunity.
HostingRepository	PRIDE
AnnounceDate	2025-04-08
AnnouncementXML	Submission_2025-04-07_23:31:03.582.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Christina Stolze
SpeciesList	scientific name: Marchantia polymorpha; NCBI TaxID: 3197;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 07:22:21	ID requested
⏵ 1	2025-04-07 23:31:03	announced

Dataset with its publication pending

submitter keyword: SERK, BIR,Marchantia polymorpha, phosphoproteomics

Hirofumi Nakagami
contact affiliation	Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact email	nakagami@mpipz.mpg.de
lab head
Sara Christina Stolze
contact affiliation	Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact email	stolze@mpipz.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053428
     1. Label: PRIDE project
     2. Name: Conserved role of the SERK‒BIR module in development and immunity across land plants