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PXD053158
PXD053158 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In vivo proteomic labeling reveals diverse proteomes for therapeutic targets |
| Description | Proteomics has played a revolutionary role in life and medical sciences. However, the barrier between isolated samples and in vivo intact microenvironment has posed a huge challenge that hinders the next-generation development of proteomics. Here, we successfully generated an in vivo visible protein labeling (IVPL) model based on a new labeling substrate set and homemade APEX2-EGFPf/f mice. The Btn-Ph-3F substrate exhibits good stability in organs possessing complex microenvironments and diverse degrading enzymes, while APEX2-EGFPf/f mice can be mated with any commercial Cre mice, enabling specific proteomic labeling for customized cell groups in distant organs in vivo. Moving beyond profiling in situ proteome for intestinal epithelium, mammary glands, and Treg cells within tumors, we further achieved labeling of trace amounts of exogenous proteomics of exosomes derived from cancer patients in live mice. Our work not only establishes an advanced model for in vivo proteomic labeling, but also profiles ultimately exosomal actors in recipient organs for targeted therapy. |
| HostingRepository | iProX |
| AnnounceDate | 2024-06-16 |
| AnnouncementXML | Submission_2025-11-30_17:48:25.682.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuening Jiang |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-16 23:04:04 | ID requested | |
| ⏵ 1 | 2025-11-30 17:48:26 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: in vivo proteomic labeling, APEX, exosomes, breast cancer, targeted therapy |
Contact List
| Mo Li | |
|---|---|
| contact affiliation | Peking University Third Hospital |
| contact email | limo@hsc.pku.edu.cn |
| lab head | |
| Yuening Jiang | |
| contact affiliation | Peking University Third Hospital |
| contact email | 2111110435@stu.pku.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
