PXD052631

PXD052631 is an original dataset announced via ProteomeXchange.

Title	G-CSF/NAMPT signaling drives neutrophil dysfunction and enhances bacterial infection susceptibility in cancer patients
Description	Despite advancements in cancer therapies, bacterial complications remain a major challenge, delaying treatment and worsening outcomes. While immunosuppressive therapies and prolonged hospitalizations contribute, they do not fully explain the elevated infection risk in cancer patients. Here we show that tumors producing high levels of granulocyte colony-stimulating factor (G-CSF) promote the persistence of Gram-negative pathogens in head and neck squamous cell carcinoma. We identify tumor-driven activation of the nicotinamide phosphoribosyltransferase (NAMPT)- nicotinamide adenine dinucleotide (NAD) signaling axis in neutrophil progenitors, resulting in impaired phagocytosis and neutrophil extracellular traps formation, accelerated neutrophil aging, and buildup of tissue-damaging neutrophil subsets. This disrupts lung tissue integrity and facilitates bacterial persistence. Importantly, targeting the G-CSF-NAMPT pathway prevents the generation of dysfunctional neutrophils and improves bacterial clearance in vivo. Our findings reveal tumor-induced, NAMPT-dependent neutrophil reprogramming as a central driver of compromised antimicrobial defenses in cancer. Therapeutic strategies aimed at modulating G-CSF-NAMPT signaling could enhance infection control and survival for cancer patients.
HostingRepository	PRIDE
AnnounceDate	2025-11-14
AnnouncementXML	Submission_2025-11-13_16:06:47.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bente Siebels
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-28 06:30:43	ID requested
⏵ 1	2025-11-13 16:06:48	announced

Dataset with its publication pending

submitter keyword: None

Prof. Dr.
contact affiliation	Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany
contact email	jadwiga.jablonska@uk-essen.de
lab head
Bente Siebels
contact affiliation	Section for Mass spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf
contact email	b.siebels@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD052631

PRIDE project URI

• PRIDE
  1. PXD052631
     1. Label: PRIDE project
     2. Name: G-CSF/NAMPT signaling drives neutrophil dysfunction and enhances bacterial infection susceptibility in cancer patients