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PXD052551

PXD052551 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferential alkylation of PHLPP2
DescriptionTo determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-step differential alkylation experiment was compared to the 1-step alkylation reactions under fully denaturing conditions with iodoacetamide and methyl methanethiosulfonate.
HostingRepositoryPRIDE
AnnounceDate2025-03-13
AnnouncementXMLSubmission_2025-03-13_02:26:01.984.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarkus Hartl
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-24 02:54:47ID requested
12025-03-13 02:26:02announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: differential alkylation,Free thiols
Contact List
Dr. Thomas A. Leonard
contact affiliationDepartment of Structural and Computational Biology, Max Perutz Labs, Campus Vienna Biocenter 5, 1030 Vienna, Austria
contact emailthomas.leonard@maxperutzlabs.ac.at
lab head
Markus Hartl
contact affiliationMax Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria
contact emailproteomics@maxperutzlabs.ac.at
dataset submitter
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Dataset FTP location
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