PXD052440 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Restriction of Arginine Induces Antibiotic Tolerance in Staphylococcus aureus |
Description | Staphylococcus aureus is responsible for a substantial number of invasive infections globally each year. These infections are problematic because they are frequently recalcitrant to antibiotic treatment. Antibiotic tolerance, the ability of bacteria to persist despite normally lethal doses of antibiotics, contributes to antibiotic treatment failure in S. aureus infections. To understand how antibiotic tolerance is induced, S. aureus biofilms exposed to multiple anti-staphylococcal antibiotics were examined using both quantitative proteomics and transposon sequencing. These screens indicated that arginine metabolism is involved in antibiotic tolerance within a biofilm and led to the hypothesis that depletion of arginine within S. aureus communities can induce antibiotic tolerance. Consistent with this hypothesis, inactivation of argH, the final gene in the arginine synthesis pathway, induces antibiotic tolerance. Arginine restriction was found to induce antibiotic tolerance via inhibition of protein synthesis. In murine skin and bone infection models, an argH mutant has enhanced ability to survive antibiotic treatment with vancomycin, highlighting the relationship between arginine metabolism and antibiotic tolerance during S. aureus infection. Uncovering this link between arginine metabolism and antibiotic tolerance has the potential to open new therapeutic avenues targeting previously recalcitrant S. aureus infections. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:49:23.572.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dale Chaput |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-21 06:39:22 | ID requested | |
1 | 2024-07-11 06:05:08 | announced | |
⏵ 2 | 2024-10-22 06:49:23 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, Skin and Soft Tissue Infection (SSTI), Biofilm, antibiotic treatment failure, TnSeq, protein synthesis,Staphylococcus aureus |
Contact List
Jeffrey Freiberg |
contact affiliation | Instructor in Medicine, Division of Infectious Diseases, Vanderbilt University |
contact email | jeffrey.freiberg@vumc.org |
lab head | |
Dale Chaput |
contact affiliation | University of South Florida |
contact email | chaput@usf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052440
- Label: PRIDE project
- Name: Restriction of Arginine Induces Antibiotic Tolerance in Staphylococcus aureus