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PXD052401

PXD052401 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein
DescriptionMicroalgae are promising production platforms for the cost-effective production of recombinant proteins. We have recently established that the red alga Porphyridium purpureum provides superior transgene expression properties, due to the episomal main- tenance of transformation vectors as multicopy plasmids in the nucleus. Here, we have explored the potential of Porphyridium to synthesize complex pharmaceutical proteins to high levels. Testing expression constructs for a candidate subunit vaccine against the hepatitis C virus (HCV), we show that the soluble HCV E2 glycoprotein can be produced in transgenic algal cultures to high levels. The antigen undergoes faithful posttranslational modification by N-glycosylation and is recognized by conformationally selective antibodies, suggesting that it adopts a proper antigenic conformation in the endoplasmic reticulum of red algal cells. We also report the experimental determina- tion of the structure of the N-glycan moiety that is attached to glycosylated proteins in Porphyridium. Finally, we demonstrate the immunogenicity of the HCV antigen produced in red algae when administered by injection as pure protein or by feeding of algal biomass.
HostingRepositoryPRIDE
AnnounceDate2024-05-21
AnnouncementXMLSubmission_2024-05-21_03:36:18.417.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLara Hoepfner
SpeciesList scientific name: Porphyridium purpureum; NCBI TaxID: 35688;
ModificationListcomplex glycosylation
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-20 02:46:39ID requested
12024-05-21 03:36:18announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: N-glycosylation,HCV, Porphyridium
Contact List
Michael Hippler
contact affiliationInstitute for plant biology and biotechnology, University of Münster
contact emailmhippler@uni-muenster.de
lab head
Lara Hoepfner
contact affiliationIBBP-Plant Biochemistry and Biotechnology AG Prof. Dr. Michael Hippler University of Münster
contact emaill_hoep03@wwu.de
dataset submitter
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Dataset FTP location
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