PXD052401 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein |
Description | Microalgae are promising production platforms for the cost-effective production of recombinant proteins. We have recently established that the red alga Porphyridium purpureum provides superior transgene expression properties, due to the episomal main- tenance of transformation vectors as multicopy plasmids in the nucleus. Here, we have explored the potential of Porphyridium to synthesize complex pharmaceutical proteins to high levels. Testing expression constructs for a candidate subunit vaccine against the hepatitis C virus (HCV), we show that the soluble HCV E2 glycoprotein can be produced in transgenic algal cultures to high levels. The antigen undergoes faithful posttranslational modification by N-glycosylation and is recognized by conformationally selective antibodies, suggesting that it adopts a proper antigenic conformation in the endoplasmic reticulum of red algal cells. We also report the experimental determina- tion of the structure of the N-glycan moiety that is attached to glycosylated proteins in Porphyridium. Finally, we demonstrate the immunogenicity of the HCV antigen produced in red algae when administered by injection as pure protein or by feeding of algal biomass. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-21 |
AnnouncementXML | Submission_2024-05-21_03:36:18.417.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lara Hoepfner |
SpeciesList | scientific name: Porphyridium purpureum; NCBI TaxID: 35688; |
ModificationList | complex glycosylation |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-20 02:46:39 | ID requested | |
⏵ 1 | 2024-05-21 03:36:18 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: N-glycosylation,HCV, Porphyridium |
Contact List
Michael Hippler |
contact affiliation | Institute for plant biology and biotechnology, University of Münster |
contact email | mhippler@uni-muenster.de |
lab head | |
Lara Hoepfner |
contact affiliation | IBBP-Plant Biochemistry and Biotechnology AG Prof. Dr. Michael Hippler University of Münster |
contact email | l_hoep03@wwu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD052401 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052401
- Label: PRIDE project
- Name: The red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein