PXD052367

PXD052367 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cysteine S-acetylation is a post-translational modification involved in metabolic regulation
Description	Cysteine is a reactive amino acid central to the catalytic activities of many enzymes and a common target of post-translational modifications (PTMs). Long-chain acyl PTMs, such as palmitoylation, can modify cysteine residues and induce changes in protein subcellular localization. We hypothesized that cysteine could also undergo short-chain acyl modifications, such as cysteine S-acetylation. To test this, we developed sample preparation and non-targeted mass spectrometry protocols to analyze the mouse liver proteome for cysteine acetylation. Our findings revealed hundreds of sites of cysteine acetylation across multiple tissue types, revealing a previously uncharacterized cysteine acetylome. Cysteine acetylation shows a marked cytoplasmic subcellular localization signature, with tissue-specific acetylome patterns and specific changes upon metabolic stress. This study uncovers a novel aspect of cysteine biochemistry, highlighting short-chain modifications alongside known long-chain acyl PTMs. These findings enrich our understanding of the landscape of acyl modifications and suggest new research directions in enzyme activity regulation and cellular signaling in metabolism.
HostingRepository	jPOST
AnnounceDate	2024-05-29
AnnouncementXML	Submission_2024-05-29_07:48:47.752.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	E. Keith Keenan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	N6-acetyl-L-lysine; S-acetyl-L-cysteine; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-18 14:55:39	ID requested
⏵ 1	2024-05-29 07:48:48	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Cysteine acetylation, Post-translational modification, Protein acetylation, Metabolism

submitter keyword: Cysteine acetylation, Post-translational modification, Protein acetylation, Metabolism

Contact List

Mathew Hirschey
lab head
E. Keith Keenan
contact affiliation	Duke University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003104/