PXD052340

PXD052340 is an original dataset announced via ProteomeXchange.

Title	Increased antioxidative defense and reduced advanced glycation end-product formation by metabolic adaptation in Non-Small-Cell-Lung-Cancer patients
Description	Oxidative stress is a critical driver of lung cancer progression, causing DNA and protein instability and supporting oncogenic transformation. On metabolic level, mediators of oxidative stress, reactive oxygen species (ROS), can oxidatively modify metabolic enzymes and reroute metabolic pathways according to tumor needs. The main carriers of redox signals are thiol groups, which due to their reactive nature are rarely analyzed in a clinical setting. To accurately address the cross talk between redox signaling and metabolism in lung cancer, we carefully collected tumor as well as neighboring healthy tissue from 70 individuals with non-small cell lung cancer: samples were collected at the point of surgical tumor removal and preserved immediately in a thiol-quenching organic solvent solution, before being subjected to redox-proteomics analysis. As a result of such an unbiased and meticulous approach we for the first time report ex vivo evidence of higher oxidation of a number of key metabolic enzymes in tumor tissue, especially those involved in glucose metabolism. We furthermore demonstrate evidence of the tumor striving to maintain functional oxidative metabolism amid the prominent rise of intracellular oxidative stress. Lastly, we report both redox and protein level deactivation of the glyoxalase system, which might be compensated by higher excretion of toxic methylglyoxal into the blood stream and/or higher GAPDH activity preserving cancer progression.
HostingRepository	PRIDE
AnnounceDate	2025-05-13
AnnouncementXML	Submission_2025-05-13_07:41:24.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Liesinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-ethylmaleimide derivatized cysteine; monohydroxylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-17 04:41:19	ID requested
⏵ 1	2025-05-13 07:41:24	announced

Dataset with its publication pending

submitter keyword: lung cacncer, oxidative stress, redox, methylglyoxal

Ruth Birner-Gruenberger
contact affiliation	Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9, 1060 Vienna, Austria; Research and Diagnostic Institute of Pathology, Medical University of Graz, Stiftingtalstraße 6, 8036, Graz, Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Laura Liesinger
contact affiliation	Faculty of Technical Chemistry, Institute of Chemical Technologies and Analytics, Technische Universität Wien (TU Wien), 1060 Vienna, Austria;
contact email	laura.liesinger@tuwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052340

PRIDE project URI

• PRIDE
  1. PXD052340
     1. Label: PRIDE project
     2. Name: Increased antioxidative defense and reduced advanced glycation end-product formation by metabolic adaptation in Non-Small-Cell-Lung-Cancer patients