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PXD052340

PXD052340 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIncreased antioxidative defense and reduced advanced glycation end-product formation by metabolic adaptation in Non-Small-Cell-Lung-Cancer patients
DescriptionOxidative stress is a critical driver of lung cancer progression, causing DNA and protein instability and supporting oncogenic transformation. On metabolic level, mediators of oxidative stress, reactive oxygen species (ROS), can oxidatively modify metabolic enzymes and reroute metabolic pathways according to tumor needs. The main carriers of redox signals are thiol groups, which due to their reactive nature are rarely analyzed in a clinical setting. To accurately address the cross talk between redox signaling and metabolism in lung cancer, we carefully collected tumor as well as neighboring healthy tissue from 70 individuals with non-small cell lung cancer: samples were collected at the point of surgical tumor removal and preserved immediately in a thiol-quenching organic solvent solution, before being subjected to redox-proteomics analysis. As a result of such an unbiased and meticulous approach we for the first time report ex vivo evidence of higher oxidation of a number of key metabolic enzymes in tumor tissue, especially those involved in glucose metabolism. We furthermore demonstrate evidence of the tumor striving to maintain functional oxidative metabolism amid the prominent rise of intracellular oxidative stress. Lastly, we report both redox and protein level deactivation of the glyoxalase system, which might be compensated by higher excretion of toxic methylglyoxal into the blood stream and/or higher GAPDH activity preserving cancer progression.
HostingRepositoryPRIDE
AnnounceDate2025-05-13
AnnouncementXMLSubmission_2025-05-13_07:41:24.386.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Liesinger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListN-ethylmaleimide derivatized cysteine; monohydroxylated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-17 04:41:19ID requested
12025-05-13 07:41:24announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: lung cacncer, oxidative stress, redox, methylglyoxal
Contact List
Ruth Birner-Gruenberger
contact affiliationInstitute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9, 1060 Vienna, Austria; Research and Diagnostic Institute of Pathology, Medical University of Graz, Stiftingtalstraße 6, 8036, Graz, Austria
contact emailruth.birner-gruenberger@tuwien.ac.at
lab head
Laura Liesinger
contact affiliationFaculty of Technical Chemistry, Institute of Chemical Technologies and Analytics, Technische Universität Wien (TU Wien), 1060 Vienna, Austria;
contact emaillaura.liesinger@tuwien.ac.at
dataset submitter
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Dataset FTP location
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