PXD052239

PXD052239 is an original dataset announced via ProteomeXchange.

Title	SNAP47 acetylation-dependent regulatory mechanism governing autophagosome-lysosome fusion by modulating the recruitment of HOPS to autophagic vacuoles
Description	Autophagy, a conserved catabolic process implicated in a diverse array of human diseases, requires efficient fusion between autophagosomes and lysosomes to function effectively. Recently, SNAP47 has been identified as a key component of the default SNARE complex mediating autophagosome-lysosome fusion in both bulk and selective autophagy. However, the spatiotemporal regulatory mechanisms of this SNARE complex remain unknown. In this study, we found that SNAP47 undergoes acetylation followed by deacetylation during bulk autophagy and mitophagy. The acetylation status of SNAP47 is regulated by the acetyltransferase CBP and the deacetylase HDAC2. Notably, the spatiotemporal regulatory dynamics of SNAP47 acetylation differ between bulk autophagy and mitophagy due to distinct regulation on the activity of acetyltransferase and deacetylase. Acetylated SNAP47 inhibits autophagosome-lysosome fusion by indirectly impeding SNARE complex assembly. Mechanistically, deacetylated SNAP47 recruits HOPS components to autophagic vacuoles, while acetylated SNAP47 inhibits this recruitment, consequently leading to the failure of SNARE complex assembly. Taken together, our study uncovers a SNAP47 acetylation-dependent regulatory mechanism governing autophagosome-lysosome fusion by modulating the recruitment of HOPS to autophagic vacuoles.
HostingRepository	iProX
AnnounceDate	2024-05-13
AnnouncementXML	Submission_2024-05-13_19:34:09.597.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haohao Tang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-13 19:33:28	ID requested
⏵ 1	2024-05-13 19:34:09	announced

Dataset with its publication pending

submitter keyword: SNAP47, autophagosome-lysosome fusion

Yang Chen
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	chenyang1816185048@bjmu.edu.cn
lab head
Haohao Tang
contact affiliation	Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University
contact email	Tanghaohao0987@163.com
dataset submitter

iProX dataset URI