PXD052188

PXD052188 is an original dataset announced via ProteomeXchange.

Title	Getting over it? A proteomic analysis of mechanisms driving multigenerational acclimation to organic ultraviolet filters in Daphnia magna
Description	Organic ultraviolet filters (UVFs) such as avobenzone, octocrylene and oxybenzone, are considered contaminants of concern due to their widespread occurrence in aquatic environments. Previous research has demonstrated that these UVFs are toxic to aquatic invertebrates over single generation exposures; however, data regarding long-term outcomes across generations of continuous exposure are lacking. Research performed in parallel to this manuscript (Boyd et al., 2023a) observed toxicity to novel UVF exposure, (> 40 % mortality, 46 % decreased reproduction); however, toxic effects were absent after 3 generations of continuous exposure. This study sought to identify the mechanisms behind this observed acclimation across generations through analysis of daphnid proteomes at the end of the F0, F1 and F3 generations. Impaired metabolism and immune response processes were observed in the F0 generation, with decreased abundance in > 80% of altered proteins in octocrylene and oxybenzone exposures. The impairments of these processes were gradually reversed over subsequent generations, with > 60 % of altered proteins demonstrating increased abundance by the F3 generation. An increase in chitin production that could reduce membrane permeability to xenobiotics and pathogens, along with enhanced immune response processes may allow exposed populations to negate many of the negative effects associated with UVF exposure. These results offer mechanistic insights into the gradual acclimation of continuously exposed D. magna populations that have been observed in response to a variety of contaminants, and further serve to highlight the importance of utilizing a long-term approach for studies seeking to model contamination risks in wild populations.
HostingRepository	PRIDE
AnnounceDate	2025-08-03
AnnouncementXML	Submission_2025-08-03_15:16:08.034.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Uhrig
SpeciesList	scientific name: Daphnia magna; NCBI TaxID: 35525;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-10 15:46:14	ID requested
⏵ 1	2025-08-03 15:16:08	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, DIA,Daphnia magna

R. Glen Uhrig
contact affiliation	Department of Biological Sciences, University of Alberta
contact email	ruhrig@ualberta.ca
lab head
Richard Uhrig
contact affiliation	University of Alberta
contact email	ruhrig@ualberta.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD052188

PRIDE project URI

• PRIDE
  1. PXD052188
     1. Label: PRIDE project
     2. Name: Getting over it? A proteomic analysis of mechanisms driving multigenerational acclimation to organic ultraviolet filters in Daphnia magna