PXD052107

PXD052107 is an original dataset announced via ProteomeXchange.

Title	Strigolactones positively regulate HY5-dependent autophagy and the degradation of ubiquitinated proteins in response to cold stress in tomato
Description	Autophagy, involved in protein degradation and amino acid recycling, plays a key role in plant development and stress responses. However, the relationship between autophagy and phytohormones is unclear. We used diverse methods, including CRISPR/Cas9, UPLC-MS/MS, chromatin immunoprecipitation, electrophoretic mobility shift assays, and dual-luciferase assays to explore the molecular mechanism of strigolactones in regulating autophagy and the degradation of ubiquitinated proteins under cold stress in tomato. We show that cold stress induced the accumulation of ubiquitinated proteins. Mutants deficient in strigolactone biosynthesis were more sensitive to cold stress with a higher accumulation of ubiquitinated proteins, while treatment with the synthetic strigolactone analog GR245DS enhanced cold tolerance in tomato, with a higher accumulation of autophagosomes and transcripts of autophagy-related genes (ATGs), and a lower accumulation of ubiquitinated proteins. Meanwhile, cold stress induced ELONGATED HYPOCOTYL 5 (HY5) accumulation, which was triggered by strigolactones. HY5 further trans-activated ATG18a promoter, resulting in autophagy formation. Mutation of ATG18a compromised strigolactone-induced cold tolerance, followed by a decreased formation of autophagosomes and an increased accumulation of ubiquitinated proteins. These findings reveal that strigolactones positively regulate autophagy in an HY5-dependent manner and promote the degradation of ubiquitinated proteins under cold conditions in tomato.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:58.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cheng Chi
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-08 02:27:41	ID requested
1	2024-07-31 19:09:49	announced
⏵ 2	2024-10-22 06:51:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: autophagy-related gene (ATG), tomato,autophagy, ELONGATED HYPOCOTYL 5 (HY5), strigolactones (SLs), cold stress

Jie Zhou
contact affiliation	Department of Horticulture, Zijingang Campus, Zhejiang University, China
contact email	jie@zju.edu.cn
lab head
Cheng Chi
contact affiliation	Peking University Institute of Advanced Agricultural Sciences
contact email	cheng.chi@pku-iaas.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052107
     1. Label: PRIDE project
     2. Name: Strigolactones positively regulate HY5-dependent autophagy and the degradation of ubiquitinated proteins in response to cold stress in tomato