PXD051888

PXD051888 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Multi-omics analysis using antibody-based in situ biotinylation technique reveals the mechanism of Cajal body formation
Description	Membrane-less subcellular compartments such as cytoplasmic bodies, nuclear bodies, and paraspeckles play important roles in a variety of cellular functions. Although many of the techniques for identifying the components of cellular bodies were developed to address their function, no technique to comprehensively identify the components of these bodies in both static and dynamic states has been established. Here, we developed an antibody-based in situ biotinylation proximity-labeling technique and succeeded in identifying the components of both static and dynamic nuclear bodies, nucleolus and γH2AX foci, respectively. Using this technique, we comprehensively identified the components of Cajal bodies (CBs), including DNAs, RNAs, and proteins, and clarified their interactome in CBs. Furthermore, we captured the dynamic change of CBs by inhibiting transcription. Our analysis revealed that nascent snRNAs transcribed in CBs play a role in CB nucleation through assembling RNA binding proteins, including frontotemporal dementia-related proteins, RBMs and HNRNPs.
HostingRepository	jPOST
AnnounceDate	2025-04-30
AnnouncementXML	Submission_2025-04-29_08:00:08.909.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidehisa Takahashi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	alpha-amino acetylated residue; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 04:49:28	ID requested
⏵ 1	2025-04-29 08:00:09	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Cajal body, biotinylation, TurboID

submitter keyword: Cajal body, biotinylation, TurboID

Contact List

Hidehisa Takahashi
lab head
Hidehisa Takahashi
contact affiliation	Yokohama city university
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003046/