PXD051884

PXD051884 is an original dataset announced via ProteomeXchange.

Title	TRAF7 determines circadian period through ubiquitination and degradation of DBP
Description	D-site binding protein, DBP, is a clock-controlled transcription factor and drives daily rhythms of physiological processes through the regulation of an array of genes harboring a DNA binding motif, D-box. DBP protein levels show a circadian oscillation with an extremely robust peak/trough ratio, but how the temporal pattern is regulated by post-translational regulation is unclear. In this study, we found that DBP protein levels are down-regulated by the ubiquitin-proteasome pathway. We screened 19 dominant-negative forms of E2 enzymes and found that UBE2G1 and UBE2T mediate the degradation of DBP. A proteomic analysis of DBP-interacting proteins and database screening identified Tumor necrosis factor Receptor-Associated Factor 7 (TRAF7), a RING-type E3 ligase, that forms a complex with UBE2G1 and/or UBE2T. Overexpression of TRAF7 down-regulated DBP protein level, while knockdown of TRAF7 up-regulated DBP in cultured cells. Knockout of TRAF7 in NIH3T3 cells revealed that TRAF7 mediates the time-of-the-day-dependent regulation of DBP levels. Furthermore, TRAF7 has a period-shortening effect on the cellular clock. Together, TRAF7 plays an important role in circadian clock oscillation through destabilization of DBP.
HostingRepository	PRIDE
AnnounceDate	2024-08-28
AnnouncementXML	Submission_2024-08-27_19:05:41.854.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hikari Yoshitane
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 04:11:16	ID requested
⏵ 1	2024-08-27 19:05:42	announced

Dataset with its publication pending

submitter keyword: TRAF7, ubiquitination, protein degradation,circadian clock, DBP

Hikari Yoshitane
contact affiliation	Circadian Clock Project, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa 2-1-6, Setagaya-ku, Tokyo, 156-8506, Japan.
contact email	yoshitane-hk@igakuken.or.jp
lab head
Hikari Yoshitane
contact affiliation	Tokyo Metropoitan Instituite of Medical Sciences
contact email	yoshitane-hk@igakuken.or.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD051884

PRIDE project URI

• PRIDE
  1. PXD051884
     1. Label: PRIDE project
     2. Name: TRAF7 determines circadian period through ubiquitination and degradation of DBP