PXD051883
PXD051883 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain |
| Description | Histone post-translational modifications play pivotal roles in eukaryotic gene expression. To date, most studies have focused on modifications in unstructured histone N-terminal tail domains and their binding proteins. However, transcriptional regulation by chromatin-effector proteins that directly recognize modifications in histone globular domains has yet to be clearly demonstrated, despite the richness of their multiple modifications. Here, we show that the ATP-dependent chromatin-remodeling BAF complex stimulates p53-dependent transcription through direct interaction with H3K56ac located on the lateral surface of the histone globular domain. Mechanistically, the BAF complex recognizes nucleosomal H3K56ac via the DPF domain in the DPF2 subunit and exhibits enhanced nucleosome-remodeling activity in the presence of H3K56ac. We further demonstrate that a defect in H3K56ac–BAF complex interaction leads to impaired p53-dependent gene expression and DNA damage responses. Our study provides direct evidence that histone globular domain modifications participate in the regulation of gene expression. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-09-30 |
| AnnouncementXML | Submission_2024-09-30_00:32:59.233.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jihyun Kim |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue; N6-acetyl-L-lysine |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-04-30 03:51:23 | ID requested | |
| ⏵ 1 | 2024-09-30 00:32:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: H3K56ac nucleosome |
| Histone globular domain |
| BAF complex |
Contact List
| Jaehoon Kim | |
|---|---|
| lab head | |
| Jihyun Kim | |
| contact affiliation | Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003064/ |
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