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PXD051860

PXD051860 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChanges in Protein Fluxes and Gene Expression in Non-Injured Muscle Tissue Distant from an Acute Myotoxic Injury in Male Mice
DescriptionThe response to acute myotoxic injury requires stimulation of local repair mechanisms in the damaged tissue. However, satellite cells in muscle distant from acute injury have been reported to enter a functional state between quiescence and active proliferation. Here, we asked whether protein flux rates are altered in muscle distant from acute local myotoxic injury and how they compare to changes in gene expression from the same tissue. Broad and significant alterations in protein turnover were observed across the proteome in the limb contralateral to injury during the first 10 days after. Interestingly, mRNA changes had almost no correlation with directly measured protein turnover rates. In summary, we show consistent and striking changes in protein flux rates in muscle tissue contralateral to myotoxic injury, with no correlation between changes in mRNA levels and protein synthesis rates. This work motivates further investigation of the mechanisms, including potential neurologic factors, responsible for this distant effect.
HostingRepositoryjPOST
AnnounceDate2024-04-30
AnnouncementXMLSubmission_2024-04-29_14:13:33.763.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlec Bizieff
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListunknown modification; unknown modification
Instrument6520A Quadrupole Time-of-Flight LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-29 14:13:30ID requested
12024-04-29 14:13:34announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Muscle Damage/Injury
In-Vivo Regeneration
Satellite Cells
Proteomics
Stable Isotope Labeling
Mass Spectrometry
Protein Turnover
Contact List
Alec Bizieff
lab head
Alec Bizieff
contact affiliationUC Berkeley
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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