PXD051671

PXD051671 is an original dataset announced via ProteomeXchange.

Title	Fish species authentication in commercial fish products using mass spectrometry and spectra library approach
Description	Seafood fraud has become a global emerging issue, threatening food security and safety. Adulteration, substitution, dilution, and incorrect labeling of seafood products are fraudulent practices that violate consumer safety. In this context, developing sensitive, robust, and high-throughput molecular tools for food and feed authentication is becoming crucial for regulatory purposes. Analytical approaches such as proteomics mass spectrometry have shown promise in detecting incorrectly labeled products. For the application of these tools, genome information is crucial, but currently, for marine species of commercial importance, such information is unavailable. However, when combining proteomic analysis with spectra library matching, commercially important fish species were successfully identified, differentiated, and quantified in pure muscle samples and mixtures, even when genome information was scarce. This study further tested the previously developed proteomic-based spectra library-based approach was further tested to differentiate 29 fish species from the North Sea in individual samples, laboratory-prepared mixtures, and commercial samples. For authenticating libraries generated from 29 fish species, fresh muscle samples from the fish samples were matched against the reference libraries. Species of the fresh fish samples were correctly authenticated using the spectra libraries generated from the 29 fish species. Furthermore, processed commercial products containing mixtures of two or three fish species were matched against these spectra libraries to test the accuracy and robustness of this method for authentication of fish species. The results indicated that the method is suitable for the authentication of fish species from highly processed samples such as fish cakes and burgers. Spectra libraries built from 29 fish species in the North Sea can efficiently tackle current and future challenges in feed and food authentication analyses when prospecting new resources in the Arctic.
HostingRepository	MassIVE
AnnounceDate	2025-06-13
AnnouncementXML	Submission_2025-06-13_04:47:14.676.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Madhushri
SpeciesList	scientific name: Gadus morhua; common name: Atlantic cod; NCBI TaxID: 8049; scientific name: Merluccius merluccius; common name: European hake; NCBI TaxID: 8063; scientific name: Pollachius virens; common name: saithe; NCBI TaxID: 8060; scientific name: Melanogrammus aeglefinus; common name: haddock; NCBI TaxID: 8056; scientific name: Pollachius pollachius; common name: pollack; NCBI TaxID: 185739; scientific name: Molva molva; common name: ling; NCBI TaxID: 163112; scientific name: Trisopterus minutus; common name: poor cod; NCBI TaxID: 80722; scientific name: Trisopterus esmarkii; common name: Norway pout; NCBI TaxID: 80723; scientific name: Micromesistius poutassou; common name: blue whiting; NCBI TaxID: 81636; scientific name: Gadiculus argenteus; common name: silvery cod; NCBI TaxID: 185737; scientific name: Clupea harengus; common name: Atlantic herring; NCBI TaxID: 7950; scientific name: Scomber scombrus; common name: Atlantic mackerel; NCBI TaxID: 13677; scientific name: Trachurus trachurus; common name: Atlantic horse mackerel; NCBI TaxID: 36212; scientific name: Microstomus kitt; common name: lemon sole; NCBI TaxID: 106175; scientific name: Pleuronectes platessa; common name: European plaice; NCBI TaxID: 8262; scientific name: Limanda limanda; common name: common dab; NCBI TaxID: 27771; scientific name: Lepidorhombus whiffiagonis; common name: megrim; NCBI TaxID: 154550; scientific name: Scophthalmus maximus; common name: turbot; NCBI TaxID: 52904; scientific name: Cyclopterus lumpus; common name: lumpfish; NCBI TaxID: 8103; scientific name: Maurolicus muelleri; common name: pearlsides; NCBI TaxID: 68502; scientific name: Ammodytes marinus; common name: lesser sand-eel; NCBI TaxID: 146480; scientific name: Anarhichas lupus; common name: Atlantic wolffish; NCBI TaxID: 8204; scientific name: Squalus acanthias; common name: spiny dogfish; NCBI TaxID: 7797; scientific name: Amblyraja radiata; common name: thorny skate; NCBI TaxID: 386614; scientific name: Argentina sphyraena; common name: European argentine; NCBI TaxID: 442169; scientific name: Callionymus lyra; common name: dragonet; NCBI TaxID: 34785; scientific name: Argentina silus; common name: greater argentine; NCBI TaxID: 446415; scientific name: Merlangius merlangus; common name: whiting; NCBI TaxID: 8058; scientific name: Eutrigla gurnardus; common name: grey gurnard; NCBI TaxID: 426098;
ModificationList	Lys->CamCys; methionine oxidation with neutral loss of 80 Da
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-23 08:55:31	ID requested
⏵ 1	2025-06-13 04:47:15	announced

no publication

submitter keyword: authentication, seafood, proteomics, tandem mass spectra, spectral libraries

Madhushri Shrikant Varunjikar
contact affiliation	INSTITUTE OF MARINE RESEARCH
contact email	madhushri.shrikant.varunjikar@hi.no
lab head
Madhushri
contact affiliation	IMR, Norway
contact email	madhushri.shrikant.varunjikar@hi.no
dataset submitter

MassIVE dataset URI

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