PXD051667

PXD051667 is an original dataset announced via ProteomeXchange.

Title	Redox control of the deubiquitinating enzyme Ubp2 regulates translation during stress
Description	Protein ubiquitination is essential to govern cells ability to cope with harmful environments by regulating many aspects of protein dynamics from synthesis to degradation. As important as the ubiquitination process, the reversal of ubiquitin chains mediated by deubiquitinating enzymes (DUBs) is critical for proper recovery from stress and re-establishment of proteostasis. Although it is known that ribosomes are decorated with K63-linked polyubiquitin (K63-ub) chains that control protein synthesis under stress, the mechanisms by which these ubiquitin chains are reversed and regulate proteostasis during stress recovery are still illusive. Here, we showed in budding yeast that the DUB Ubp2 is redox regulated during oxidative stress in a reversible manner, which determines the levels of K63-ub chains present on ribosomes. We also demonstrate that Ubp2 is a processive enzyme whose activity is modulated by a series of repeated domains and the formation of important disulfide bonds. By combining, cellular, biochemical, and proteomics analyses, we showed that Ubp2 is crucial for restoring translation after stress cessation, indicating an important role in determining cellular response to oxidative stress. Our work demonstrates a novel role for Ubp2, revealing that a range of signaling pathways can be controlled by redox regulation of DUB activity in eukaryotes, which in turn will define cellular states of health and diseases.
HostingRepository	MassIVE
AnnounceDate	2025-10-26
AnnouncementXML	Submission_2025-10-26_14:40:38.963.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gustavo Silva
SpeciesList	scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationList	unknown modification
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-23 07:34:41	ID requested
⏵ 1	2025-10-26 14:40:39	announced

no publication

submitter keyword: yeast, DUBs, ubiquitination, oxidative stress, recovery

Gustavo Monteiro Silva
contact affiliation	Duke University
contact email	gustavo.silva@duke.edu
lab head
Gustavo Silva
contact affiliation	Duke University
contact email	gustavo.silva@duke.edu
dataset submitter

MassIVE dataset URI

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