PXD051654

PXD051654 is an original dataset announced via ProteomeXchange.

Title	Characterization of dsRNA Binding Proteins through Solubility Analysis Identifies ZNF385A as a dsRNA Homeostasis Regulator
Description	Double-stranded RNA (dsRNA) binding proteins (dsRBPs) play crucial roles in various cellular processes, especially in the innate immune response. Comprehensive characterization of dsRBPs is essential to understand the intricate mechanisms for dsRNA sensing and response. Traditional methods have predominantly relied on affinity purification, favoring the isolation of strong dsRNA binders. Here, we adopt the proteome integral solubility alteration (PISA) workflow for characterizing dsRBPs, resulting in the observation of 18 known dsRBPs and the identification of 200 potential dsRBPs. Next, we focus on zinc finger protein 385A (ZNF385A) and discover that its knockout activates the transcription of interferon-βin the absence of immunogenic stimuli. The knockout of ZNF385A elevates the level of endogenous dsRNAs, especially transcripts associated with retroelements, such as short interspersed nuclear element (SINE), long interspersed nuclear element (LINE), and long terminal repeat (LTR). Moreover, loss of ZNF385A enhances the bioactivity of 5-Aza-2'-deoxycytidine (5-AZA-CdR) and tumor-killing effect of NK cells. Our findings greatly expand the dsRBP reservoir and contribute to the understanding of cellular dsRNA homeostasis.
HostingRepository	iProX
AnnounceDate	2024-04-23
AnnouncementXML	Submission_2025-03-06_19:35:12.004.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiang Na
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-22 23:42:02	ID requested
1	2025-03-05 18:40:47	announced
⏵ 2	2025-03-06 19:35:12	announced	2025-03-07: Update information.

Dataset with its publication pending

submitter keyword: dsRNA, dsRBP, mass spectrometry, proteome integral solubility alteration, proteomics

Ruibing Chen
contact affiliation	Tianjin University
contact email	rbchen@tju.edu.cn
lab head
Jiang Na
contact affiliation	Tianjin University
contact email	16622080827@163.com
dataset submitter

iProX dataset URI