PXD051484

PXD051484 is an original dataset announced via ProteomeXchange.

Title	Global lysine succinylation analysis unveils post-translational regulation effect on phenylpropanoids metabolism remodeling during Lonicera japonica flowering development
Description	Lonicera japonica Thunb is a typical Chinese medicinal material with the homology of medicine and food. Post-translational modification (PTM) of proteins can significantly affect protein structure and function, and plays a very important regulatory role in plant development. However, to date, little is known about the functions of PTM in L. japonica. Therefore, the study of lysine succinylation during the floral developmental stages will help to understand the mechanism of the secondary metabolism of L. japonica. In this study, we first analyzed lysine succinylation exist in L. japonica, and found lysine succinylation is highly abundant in juvenile bud stage than gold flowering stage. Then a highly efficient enrichment method for succinylation peptides was used to identify 586 succinylation modification sites in 303 proteins in L. japonica. Our bioinformatics analysis further revealed that the succinylated proteins are involved in many important biological processes including TCA, amino acid metabolism and secondary metabolism. With the development of flowering stage of L. japonica, the succinylation degree of aspartate transaminase was up-regulated while 4-coumarate-CoA ligase was down-regulated, suggesting that succinylation of proteins is important for the bioactive compounds’ biosynthesis process in L. japonica. Protein modification in vitro also verified the impact of succinylation on both 4CL and AT enzyme activity would be changed. As a consequence, current examine provides new insight into the molecular mechanism underlying the accumulation of active ingredients in L. japonica at the post-translational modification level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:41:26.686.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Chen
SpeciesList	scientific name: Lonicera japonica; NCBI TaxID: 105884;
ModificationList	succinylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-16 03:47:55	ID requested
1	2024-05-22 22:47:40	announced
⏵ 2	2024-10-22 06:41:27	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Lonicera japonica Thunb, nano LC-MS/MS, lysine succinylation

Bingxian Yang
contact affiliation	College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, China
contact email	xianyb@zju.edu.cn
lab head
Yao Chen
contact affiliation	Zhejiang Sci-Tech University
contact email	202120801007@mails.zstu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051484
     1. Label: PRIDE project
     2. Name: Global lysine succinylation analysis unveils post-translational regulation effect on phenylpropanoids metabolism remodeling during Lonicera japonica flowering development