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PXD051387

PXD051387 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFTO deficiency in older livers exacerbates ferroptosis during ischaemia/reperfusion injury by upregulating ACSL4 and TFRC
DescriptionOlder livers are more prone to hepatic ischaemia/reperfusion injury (HIRI), which severely limits their utilization in liver transplantation (LT); however, the potential mechanism remains unclear. Here, we demonstrated older livers exhibit a higher degree of ferroptosis during HIRI. Inhibiting ferroptosis significantly attenuated older HIRI. Mass spectrometry revealed fat mass and obesity-associated gene (FTO) was downregulated in older livers, especially during HIRI. Overexpressing FTO ameliorated older HIRI by inhibiting ferroptosis. Mechanistically, ACSL4 and TFRC, two key positive contributors of ferroptosis, were targets of FTO. The mitigating effect of FTO on older HIRI required the inhibition of Acsl4 and Tfrc mRNA stability in a m6A-dependent manner. Furthermore, we demonstrated nicotinamide mononucleotide (NMN) could upregulate FTO demethylase activity to suppress ferroptosis and attenuate older HIRI. Collectively, these findings revealed an FTO-ACSL4/TFRC regulatory pathway that contributes to the pathogenesis of older HIRI, providing insight into the clinical translation of strategies related to the demethylase activity of FTO in order to improve graft function after older donor LT.
HostingRepositoryiProX
AnnounceDate2024-04-12
AnnouncementXMLSubmission_2024-04-12_02:05:31.102.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXijing Yan
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-12 02:05:06ID requested
12024-04-12 02:05:31announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: FTO deficiency, older livers exacerbates ferroptosis
Contact List
Yang Yang
contact affiliationThe Third Affiliated Hospital of Sun Yat-sen University
contact emailyysysu@163.com
lab head
Xijing Yan
contact affiliationThe Third Affiliated Hospital of Sun Yat-sen University
contact emailyanxj07@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI