PXD051349

PXD051349 is an original dataset announced via ProteomeXchange.

Title	Exosomes derived from syncytia induced by SARS-2-S promote the proliferation and metastasis of hepatocellular carcinoma cells
Description	Introduction: Coronavirus disease 2019 (COVID-19) is characterized by fever, fatigue, dry cough, dyspnea, mild pneumonia and acute lung injury (ALI), which can lead to acute respiratory distress syndrome (ARDS), and SARS-CoV-2 can accelerate tumor progression. However, the reason for the increased mortality in cancer patients infected with COVID-19 is unclear. Methods: Colony formation and wound healing assays were performed on Huh-7 cells cocultured with syncytia. Exosomes were extracted from the cell supernatant and verified by nanoparticle tracking analysis (NTA), Western blot (WB) analysis and scanning electron microscopy (SEM). Proteomic sequencing was used to analyze differentially expressed proteins in syncytia-derived exosomes (Syn-Exos) and their functions. Syn-Exo-mediated promotion of hepatocellular carcinoma cells was verified by CCK-8 and Transwell migration assays. The mechanism by which Syn-Exos promote tumor growth was analyzed by Western blotting. A patient-derived xenotransplantation (PDX) mouse model was constructed to evaluate the pathological role of the SARS-CoV-2 spike protein (SARS-2-S). The number of syncytia in the tumor tissue sections was determined by immunofluorescence analysis. Results: Syncytium formation promoted the proliferation and migration of hepatocellular carcinoma cells. Proteomic analysis revealed that proteins that regulate cell proliferation and metastasis in Syn-Exos were significantly upregulated. Syn-Exos promoted hepatocellular carcinoma cell proliferation and migration through the JAK1/STAT3 pathway. Animal experiments showed that a pseudotyped lentivirus bearing SARS-2-S (SARS-2-Spp) promoted tumor development in PDX mice. More syncytia were found in tumor tissue from SARS-2-Spp mice than from VSV-Gpp mice. Conclusions: We identified a new mechanism by which patients with COVID-19 develop liver cancer. Syn-Exos induced by SARS-2-S can promote the proliferation and metastasis of hepatocellular carcinoma cells
HostingRepository	iProX
AnnounceDate	2024-04-10
AnnouncementXML	Submission_2024-05-08_18:48:59.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haotian Lin
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-11 00:14:01	ID requested
⏵ 1	2024-05-08 18:49:00	announced

Dataset with its publication pending

submitter keyword: SARS-2-S, Syncytia, Exosomes, Hepatocellular Carcinoma, proliferation, metastasis

Congwen Wei
contact affiliation	Beijing Institute of Biotechnology
contact email	weicw@yahoo.com
lab head
Haotian Lin
contact affiliation	Beijing Institute of Biotechnology
contact email	linhaotian0703@163.com
dataset submitter

iProX dataset URI