PXD051328

PXD051328 is an original dataset announced via ProteomeXchange.

Title	Fluorescence imaging sheds light on the immune evasion mechanisms of hepatic stellate cells mediated by superoxide anion
Description	Whether and how the reactive oxygen species generated by hepatic stellate cells (HSCs) promote immune evasion of hepatocellular carcinoma (HCC) remains mysteriouss. Therefore, investigating the function of superoxide anion, the firstly generated reactive oxygen species, during the immune evasion become necessary. In this work, we establish a novel in situ imaging method for visualization of superoxide anion changes in HSCs based on a new two-photon fluorescence probe TPH. TPH comprises recognition group for superoxide anion and HSCs targeting peptides. We observe that superoxide anion in HSCs gradually rose, impairing the infiltration of CD8+ T cells in HCC mice. Further studies reveal that the cyclin-dependent kinase 4 is deactivated by superoxide anion, and then cause the up-regulation of PD-L1. Our work provides molecular insights into HSC-mediated immune evasion of HCC, which may represent potential targets for HCC immunotherapy.
HostingRepository	iProX
AnnounceDate	2024-04-08
AnnouncementXML	Submission_2024-04-10_01:36:12.627.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Tang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-10 01:35:48	ID requested
⏵ 1	2024-04-10 01:36:12	announced

Dataset with its publication pending

submitter keyword: superoxide anion, fluorescence imaging, immune evasion, hepatic stellate cells

Bo Tang
contact affiliation	College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Shandong Normal University
contact email	tangb@sdnu.edu.cn
lab head
Bo Tang
contact affiliation	College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Shandong Normal University
contact email	tangb@sdnu.edu.cn
dataset submitter

iProX dataset URI