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PXD051278

PXD051278 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSUN5 interacts with nuclear membrane LaminB1 and cytoskeletal GTPase Septin12 mediating the sperm head-and-tail junction
DescriptionAcephalic spermatozoa syndrome (ASS) is a severe teratospermia with decaudated, decapitated and malformed sperm, resulting in male infertility. Nuclear envelop (NE) protein SUN5 localizes to the junction between the sperm head and tail. Mutations in SUN5 gene have been identified most frequently (33-47%) in ASS cases, and the molecular mechanism needs to be explored. In the present study, we generated Sun5 knockout mice, which presented the phenotype of ASS. Nuclear membrane protein LaminB1, cytoskeletal GTPases Septin12 and Septin2 were identified as potential partners for interacting with SUN5 in mouse testis by immunoprecipitation-mass spectrometry (IP-MS). Further studies demonstrated that SUN5 co-operated with LaminB1, and connected proximal centriole by interacting with Septin12. The binding between SUN5 and Septin12 promoted the aggregation of SUN5 and Septin12 in the sperm neck. The disruption of LaminB1/SUN5/Septin12 complex by Sun5 deficiency caused separation of the Septin12- proximal centriole from the nucleus, leading to the breakage of the head-to-tail junction. Collectively, these data provide new insights into the pathogenesis of ASS caused by SUN5 deficiency.
HostingRepositoryiProX
AnnounceDate2024-04-08
AnnouncementXMLSubmission_2024-05-20_00:17:19.921.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYunfei Zhang
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-08 19:13:10ID requested
12024-05-20 00:17:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SUN5, LaminB1, Septin12, proximal centriole, sperm head-to-tail junction
Contact List
Xiaowei Xing
contact affiliationThe Third Xiangya Hospital of Central South University
contact emaildavy2222@163.com
lab head
Yunfei Zhang
contact affiliationThe Third Xiangya Hospital of Central South University
contact email792728211@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI