PXD051223

PXD051223 is an original dataset announced via ProteomeXchange.

Title	metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions
Description	Purple phototrophic bacteria (PPB) naturally accept CO2 into their metabolism as a primary redox sink system in photo-heterotrophy. Dedicated use of this feature for developing sustainable processes (e.g., through negative-emissions photo-bioelectrosynthesis) requires a deep knowledge of the inherent metabolic mechanisms. Here we provide evidence of the tuning of the PPB metabolic mechanisms upon redox stressing through negative polarization (-0.4 and -0.8 V vs. Ag/AgCl) in photo-bioelectrochemical devices. Using metaproteomic analysis at both reactor ans species level, we showed that a mixed PPB-culture up-regulates its ability to capture CO2 from organics oxidation through the Calvin-Besson-Bassam cycle and anaplerotic pathways, and the redox imbalance is promoted to polyhydroxyalkanoates production. The ecological relationship of PPB with mutualist bacteria stabilizes the system and opens the door for future development of photo-bioelectrochemical devices focused on CO2 up-cycling.
HostingRepository	PRIDE
AnnounceDate	2024-10-29
AnnouncementXML	Submission_2024-10-29_11:42:18.295.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Baptiste Leroy
SpeciesList	scientific name: Rhodobacter capsulatus; NCBI TaxID: 1061;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-05 10:08:03	ID requested
⏵ 1	2024-10-29 11:42:18	announced

Dataset with its publication pending

submitter keyword: Purple Bacteria

photoheterrotrophy

Bioelectrochemical system

metaproteomic

LC-MSMS

TripleTof

Leroy Baptiste
contact affiliation	Laboratory of proteomic and microbiology, University of Mons, Belgium
contact email	baptiste.leroy@umons.ac.be
lab head
Baptiste Leroy
contact affiliation	University of Mons
contact email	baptiste.leroy@umons.ac.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051223
     1. Label: PRIDE project
     2. Name: metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions