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PXD051075

PXD051075 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleConventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure
DescriptionWe developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human cells.
HostingRepositoryPRIDE
AnnounceDate2024-04-12
AnnouncementXMLSubmission_2024-04-12_12:46:17.469.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrew Michael
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue; phosphorylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-03-28 18:43:02ID requested
12024-04-12 12:46:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: in situ XL-MS, formaldehyde fixaiton, crosslinking mass spectrometry
Contact List
David C. Schriemer
contact affiliationDepartment of Chemstry & Department of Biochemistry and Molecular Biology, University of Clagary, Canada
contact emaildschriem@ucalgary.ca
lab head
Andrew Michael
contact affiliationUniversity of Calgary
contact emailandrew.michael@ucalgary.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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