PXD051075 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Conventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure |
Description | We developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-04-12 |
AnnouncementXML | Submission_2024-04-12_12:46:17.469.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrew Michael |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue; phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-03-28 18:43:02 | ID requested | |
⏵ 1 | 2024-04-12 12:46:17 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: in situ XL-MS, formaldehyde fixaiton, crosslinking mass spectrometry |
Contact List
David C. Schriemer |
contact affiliation | Department of Chemstry & Department of Biochemistry and Molecular Biology, University of Clagary, Canada |
contact email | dschriem@ucalgary.ca |
lab head | |
Andrew Michael |
contact affiliation | University of Calgary |
contact email | andrew.michael@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD051075
- Label: PRIDE project
- Name: Conventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure