PXD050921

PXD050921 is an original dataset announced via ProteomeXchange.

Title	SWI/SNF is a gatekeeper of chromatin access at enhancer networks controlling triple-negative breast cancer progression
Description	Triple-negative breast cancer (TNBC) is a particularly aggressive subtype of breast cancer with high metastatic potential, limited treatment options and low patient survival rates. By combining functional proteomics and genomics approaches, we identify an oncogenic transcriptional network in mesenchymal and invasive TNBC involving the glucocorticoid receptor (GR), GATA6, MYC and AP-1 transcription factors. Although these transcription factors bind extensively to shared enhancers, they utilize different enhancer repertoires from this shared enhancer pool to drive distinct downstream oncogenic pathways. We demonstrate that the SWI/SNF chromatin remodeling complex is a common gatekeeper of chromatin access for these transcription factors, and SWI/SNF inhibition decommissions the distinct enhancer networks and pro-tumorigenic gene programs they control. Consistently, SWI/SNF inhibition blocks growth, glucocorticoid-induced chemoresistance and invasion/metastasis. Thus, we propose that SWI/SNF-mediated chromatin remodeling drives multiple hallmarks of TNBC progression, positioning SWI/SNF as a promising therapeutic target in this disease.
HostingRepository	PRIDE
AnnounceDate	2025-08-27
AnnouncementXML	Submission_2025-08-27_03:51:57.599.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tina Ravnsborg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-23 01:22:51	ID requested
⏵ 1	2025-08-27 03:51:57	announced

Dataset with its publication pending

submitter keyword: LC-MSMS,breast cancer, human cell lines

Rasmus Siersbaek
contact affiliation	department of biochemistry and Molecular Biology. University of Southern Denmark
contact email	Siersbaek@bmb.sdu.dk
lab head
Tina Ravnsborg
contact affiliation	Department of Biochemisrty and Molecular Biology, University of Southern Denmark
contact email	tinaravnsborg@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD050921

PRIDE project URI

• PRIDE
  1. PXD050921
     1. Label: PRIDE project
     2. Name: SWI/SNF is a gatekeeper of chromatin access at enhancer networks controlling triple-negative breast cancer progression