PXD050719

PXD050719 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Actin-nucleation promoting factor N-WASP influences alpha-synuclein condensates and pathology
Description	Abnormal intraneuronal accumulation of soluble and insoluble alpha-synuclein (alpha-Syn) is one of the main pathological hallmarks of synucleinopathies, such as Parkinson’s disease (PD). It has been well- documented that the reversible liquid-liquid phase separation of alpha-Syn can modulate synaptic vesicle condensates at the presynaptic terminals. However, alpha-Syn can also form liquid-like droplets that may convert into amyloid-enriched hydrogels or fibrillar polymorphs under stressful conditions. To advance our understanding on the mechanisms underlying alpha-Syn phase transition, we employed a series of unbiased proteomic analyses and found that actin and actin regulators are part of the alpha-Syn interactome. We focused on Neural Wiskott-Aldrich syndrome protein (N-WASP) because of its association with a rare early-onset familial form of PD. In cultured cells, we demonstrate that N-WASP undergoes phase separation and can be recruited to synapsin-1 liquid-like droplets, whereas it is excluded from alpha-Syn/synapsin-1 condensates. Consistently, we provide evidence that wsp-1/WASL loss of function (lof) alters the number and dynamics of alpha-Syn inclusions in the nematode Caenorhabditis elegans. Together, our findings indicate that N-WASP expression may create permissive conditions that promote alpha-Syn condensates and their potentially deleterious conversion into toxic species.
HostingRepository	jPOST
AnnounceDate	2025-03-22
AnnouncementXML	Submission_2025-03-21_08:00:04.858.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enzo Scifo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-18 04:48:46	ID requested
⏵ 1	2025-03-21 08:00:05	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: alpha-synuclein
Caenorhabditis elegans
liquid-liquid phase separation
Neural Wiskott-Aldrich syndrome protein (N-WASP)

submitter keyword: alpha-synuclein

Caenorhabditis elegans

liquid-liquid phase separation

Neural Wiskott-Aldrich syndrome protein (N-WASP)

Contact List

Dr. Daniele Bano
lab head
Enzo Scifo
contact affiliation	DZNE
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002992/