PXD050423

PXD050423 is an original dataset announced via ProteomeXchange.

Title	Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin
Description	The experimental project studied a MIDAS adhesin minus mutant of predatory bacterium B. bacteriovorus.The predatory bacterium normally invades and lives inside E.coli bacteria, rounding them up to form a two-bacterial structure, called a bdelloplast, and killing the E.coli from the inside. However the MIDAS mutant predator failed to invade in 10% of cases due to one of its (many) attachment/invasion mechanisms being absent. We enriched and purified the 10% of bdelloplasts which did not have an invaded predator inside, by Percoll gradient centrifugation. Although these bdelloplasts did not have an invaded predator they were still rounded and dead. We sent the bdelloplast sample for total protein content analysis at the Oxford Advanced Proteomics Facility. We found that although the bdelloplasts areE.coli cells they also contain secreted Bdellovibrio proteins that normally an invading wild type Bdellovibrio is known to secrete into their prey, during invasion. This suggests that a short-lived failed attachment allowed the Bdellovibrio to secrete in predatory proteins , even though it failed to enter the E.coli, and that those predatory proteins alone were enough to round and kill it.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:33:47.323.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marjorie Fournier
SpeciesList	scientific name: Bdellovibrio bacteriovorus HD100; NCBI TaxID: 264462;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-06 15:53:23	ID requested
1	2024-03-18 03:17:28	announced
⏵ 2	2024-10-22 06:33:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Proteomics, Ecoli, empty bdelloplasts, B. bacteriovorus

Elizabeth Sockett
contact affiliation	Nottingham University
contact email	liz.sockett@nottingham.ac.uk
lab head
Marjorie Fournier
contact affiliation	University of Oxford
contact email	marjorie.fournier@bioch.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD050423
     1. Label: PRIDE project
     2. Name: Prey killing without invasion by Bdellovibrio bacteriovorus defective for a MIDAS-family adhesin