PXD050163
PXD050163 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Vaccine priming of rare HIV broadly neutralizing antibody precursors in nonhuman primates |
| Description | Germline-targeting immunogens hold promise for initiating the induction of broadly neutralizing antibodies (bnAbs) to human immunodeficiency virus (HIV) and other pathogens. However, antibody-antigen recognition is typically dominated by heavy chain complementarity determining region 3 (HCDR3) interactions, and vaccine priming of HCDR3-dominant bnAbs by germline-targeting immunogens has not been demonstrated in humans or outbred animals. We found that immunization with N332-GT5, an HIV envelope trimer designed to target precursors of the HCDR3-dominant bnAb BG18, primed bnAb-precursor B cells in 8 of 8 rhesus macaques to substantial frequencies, and with diverse lineages, in germinal center and memory B cells. We confirmed bnAb-mimicking, HCDR3-dominant, trimer-binding interactions with cryo-electron microscopy. The results demonstrate proof of principle for priming of HCDR3-dominant bnAb precursors in outbred animals, suggest that N332-GT5 has promise to induce similar responses in humans, and encourage application of HCDR3-dominant germline-targeting for other bnAbs to HIV and additional pathogens. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-05-03 |
| AnnouncementXML | Submission_2024-05-03_10:52:19.884.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sabyasachi Baboo |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human immunodeficiency virus; NCBI TaxID: 12721; |
| ModificationList | Oxidation; 2-pyrrolidone-5-carboxylic acid (Gln); HexNAc; 1x(18)O labeled deamidated L-asparagine |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-02-26 16:30:42 | ID requested | |
| ⏵ 1 | 2024-05-03 10:52:20 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: DeGlyPHER, N-glycans, HIV Env trimer, vaccine, germline targeting, V3-glycan epitope, bNAb |
Contact List
| John R. Yates III | |
|---|---|
| contact affiliation | The Scripps Research Institute |
| contact email | jyates@scripps.edu |
| lab head | |
| Sabyasachi Baboo | |
| contact affiliation | The Scripps Research Institute, La Jolla, CA |
| contact email | sbaboo@scripps.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v07/MSV000094174/ |
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