PXD050098
PXD050098 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Hydrophilic interaction chromatography coupled to ultraviolet photodissociation affords identification, localization, and relative quantitation of glycans on intact glycoproteins |
Description | Protein glycosylation is implicated in a wide array of diseases, yet glycoprotein analysis remains elusive owing to the extreme heterogeneity of glycans including microheterogeneity at the same amino acid residue (glycosite). Top-down mass spectrometry (MS) allows precise identification and localization of glycans on intact proteins, and coupling top-down MS with chromatography allows time-resolved characterization of glycoforms. Here, we couple ultraviolet photo-dissociation (UVPD) to hydrophilic interaction chromatography (HILIC) to advance the characterization of glycoproteins ranging from 15-34 kDa, offering site localization of glycans, providing sequence coverages up to 93% and relative quan-titation of individual glycoforms. |
HostingRepository | jPOST |
AnnounceDate | 2024-02-24 |
AnnouncementXML | Submission_2024-02-23_21:10:18.720.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Virginia James |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxymethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Gln) |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-23 19:56:22 | ID requested | |
⏵ 1 | 2024-02-23 21:10:19 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Protein glycosylation, top-down mass spectrometry, ultraviolet photodissociation |
Contact List
Jennifer Brodbelt | |
---|---|
lab head | |
Virginia James | |
contact affiliation | UT-Austin |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002959/ |