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PXD050098

PXD050098 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHydrophilic interaction chromatography coupled to ultraviolet photodissociation affords identification, localization, and relative quantitation of glycans on intact glycoproteins
DescriptionProtein glycosylation is implicated in a wide array of diseases, yet glycoprotein analysis remains elusive owing to the extreme heterogeneity of glycans including microheterogeneity at the same amino acid residue (glycosite). Top-down mass spectrometry (MS) allows precise identification and localization of glycans on intact proteins, and coupling top-down MS with chromatography allows time-resolved characterization of glycoforms. Here, we couple ultraviolet photo-dissociation (UVPD) to hydrophilic interaction chromatography (HILIC) to advance the characterization of glycoproteins ranging from 15-34 kDa, offering site localization of glycans, providing sequence coverages up to 93% and relative quan-titation of individual glycoforms.
HostingRepositoryjPOST
AnnounceDate2024-02-24
AnnouncementXMLSubmission_2024-02-23_21:10:18.720.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVirginia James
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxymethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Gln)
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-23 19:56:22ID requested
12024-02-23 21:10:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Protein glycosylation, top-down mass spectrometry, ultraviolet photodissociation
Contact List
Jennifer Brodbelt
lab head
Virginia James
contact affiliationUT-Austin
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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