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PXD050051

PXD050051 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion
DescriptionEmbryonic stem cells (ESCs) were labeled with heavy isotopes, 13C615N4-arginine (Arg10) and 13C614N2-lysine (Lys6), or light isotopes, 12C614N4-arginine (Arg0) and 12C614N2-lysine (Lys0), respectively, for a total of 7 passages to ensure efficient labeling (>97%). The ESCs labeled with heavy isotopes were then treated with 20 mM SO for 24 hours before harvesting cells. Protein lysates of control and SO treated ESCs were prepared and mixed equivalently. Subsequently, trypsin digestion, high-performance liquid chromatography (HPLC) fractionation, Kla peptide enrichment with immobilized anti-Kla antibody, and high-resolution liquid chromatography–tandem MS (LC-MS/MS) were implemented. The resulting MS/MS data were processed using Maxquant search engine (v.1.5.2.8).
HostingRepositoryiProX
AnnounceDate2024-02-22
AnnouncementXMLSubmission_2024-05-05_21:11:25.362.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQiman Dong
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListlactic acid
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-22 18:11:46ID requested
⏵ 12024-05-05 21:11:25announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Lactylation, Embryonic stem cells
Contact List
Lingyi Chen
contact affiliationNankai University
contact emaillingyichen@nankai.edu.cn
lab head
Qiman Dong
contact affiliationNankai University
contact email18330225980@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI

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