PXD050024

PXD050024 is an original dataset announced via ProteomeXchange.

Title	Do extracellular vesicles have specific target cells? Extracellular Vesicle mediated embryo maternal communication
Description	Extracellular vesicles (EVs) have emerged as key messengers in intercellular communications. However, the precise mechanisms of how recipient cells read EV messages are not clear. In this study, we investigated the roles of EV protein cargo, EV parent cell type, and recipient cell type in EV function using a human embryo implantation model. The JAr cell line served as an analog of human trophoblast cells, and EVs isolated from these cells were introduced into receptive endometrial epithelial cell analog RL95-2 cells to simulate EV-mediated embryo-maternal communication. Downregulation of Zinc-finger protein 81 (ZNF81) gene expression in RL95-2 cells in response to trophoblast EVs were used as a readout of JAr EV functionality. EVs from the non-trophoblastic cell line (HEK-293 cells) were used as a control to assess the specificity of trophoblast EV functionality in endometrial epithelial cells. Our results demonstrated that ZNF81 gene downregulation is a specific response of RL95-2 cells to JAr EVs, as HEK-293 cell EVs did not induce any gene expression changes. Furthermore, we conducted an analysis of the proteomic cargo profiles of JAr and HEK-293 cell EVs. Interestingly, JAr EVs exhibited a unique proteomic cargo profile compared to that of HEK EVs. Some of the proteins uniquely identified in JAr EVs are also known to play roles in embryo implantation. Moreover, JAr EVs were specifically enriched with important transcription factors that regulate ZNF81 gene expression. These findings contribute to our understanding of the specificity of EV functionality and cellular response in the context of embryo implantation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:48:46.547.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SUBHASHINI MUHANDIRAM MUHANDIRAM RALALAGE
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-22 02:08:28	ID requested
1	2024-07-03 02:58:32	announced
⏵ 2	2024-10-22 06:48:46	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: JAr cell line,Extracellular Vesicles, embryo implantation, HEK 293 cell line

Alireza Fazeli
contact affiliation	Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences
contact email	alireza.fazeli@emu.ee
lab head
SUBHASHINI MUHANDIRAM MUHANDIRAM RALALAGE
contact affiliation	Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences
contact email	soomuhandiram2@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD050024

PRIDE project URI

• PRIDE
  1. PXD050024
     1. Label: PRIDE project
     2. Name: Do extracellular vesicles have specific target cells? Extracellular Vesicle mediated embryo maternal communication