PXD049995

PXD049995 is an original dataset announced via ProteomeXchange.

Title	TblncRNA-23, a long non-coding RNA transcribed by RNA polymerase I, regulates developmental changes in Trypanosoma brucei
Description	The two major surface proteins of procyclic forms in the insect midgut, EP and GPEET procyclin, are transcribed from a polycistronic transcription unit by RNA polymerase I. We identified a long non-coding RNA, termed TblncRNA-23, that is encoded between the two procyclin genes. TblncRNA-23 was localized to the nucleolus and also associated with polysomes. Overexpression of TblncRNA-23 identified EP and GPEET mRNAs as targets, as well as other mRNAs, that changed abundance in the transition from early to late procyclic forms. TblncRNA-23 interacted with its substrates via base-pairing using different domains. Purification of TblncRNA-23-associated proteins by RaPID identified hundreds of proteins, including proteins translated from its target mRNAs, suggesting association with translating ribosomes. Nucleolar TblncRNA-23 associated with multiple pre-rRNA processing factors, and cytoplasmic TblncRNA-23 with RNA binding proteins that regulate mRNA stability and translation. Early and late procyclic forms differ in their social motility (SoMo) capabilities, which is essential for migration away from the insect midgut to enable parasite transmission. Overexpression of TblncRNA-23 resulted in parasites exhibiting hyperSoMo, suggesting a regulatory role in SoMo and the parasite's potential to cycle between hosts
HostingRepository	PRIDE
AnnounceDate	2025-04-06
AnnouncementXML	Submission_2025-04-05_22:50:06.577.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-21 06:55:25	ID requested
⏵ 1	2025-04-05 22:50:06	announced

Dataset with its publication pending

submitter keyword: Trypanosoma brucei,long non-coding RNA

Shulamit Michaeli
contact affiliation	Everard Goodman Faculty of Life Sciences Nanomaterials Research Center Institute of Nanotechnology & Advanced Materials Bar-Ilan University, Ramat-Gan 52900, Israel
contact email	michaes@biu.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD049995
     1. Label: PRIDE project
     2. Name: TblncRNA-23, a long non-coding RNA transcribed by RNA polymerase I, regulates developmental changes in Trypanosoma brucei