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PXD049969

PXD049969 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInfluence of Different Sample Preparation Approaches on Proteoform Identification by Top-Down Proteomics
DescriptionTop-down proteomics (TDP) has made significant advances in the past, and a plethora of sample preparation workflows have been developed. Here, we systematically investigated the influence of different sample preparation steps on proteoform and protein identifications, including cell lysis, reduction and alkylation, proteoform enrichment, purification, and fractionation. We found that all steps in sample preparation influence the subset of proteoforms identified, e.g., their number, confidence, physicochemical properties, and artificially generated modifications. The various sample preparations resulted in complementary identifications, significantly increasing the depth of the analysis. Overall, 15,089 proteoforms from 2,780 protein accessions of human Caco-2 cells were identified, providing the most comprehensive dataset of this cell line up to now. The results from our study can serve as a guideline for designing and adapting TDP sample preparation strategies to particular research questions.
HostingRepositoryPRIDE
AnnounceDate2024-08-08
AnnouncementXMLSubmission_2024-08-08_05:03:12.382.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Tholey
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-02-20 11:14:34ID requested
12024-08-08 05:03:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Top-Down Proteomics
Proteoforms
Sample Preparation
Fractionation
Mass spectrometry.
Contact List
Andreas Tholey
contact affiliationSystematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, 24105 Kiel, Germany
contact emaila.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliationSystematic Proteome Research & Bioanalytics, University of Kiel
contact emaila.tholey@iem.uni-kiel.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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