PXD049969 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Influence of Different Sample Preparation Approaches on Proteoform Identification by Top-Down Proteomics |
Description | Top-down proteomics (TDP) has made significant advances in the past, and a plethora of sample preparation workflows have been developed. Here, we systematically investigated the influence of different sample preparation steps on proteoform and protein identifications, including cell lysis, reduction and alkylation, proteoform enrichment, purification, and fractionation. We found that all steps in sample preparation influence the subset of proteoforms identified, e.g., their number, confidence, physicochemical properties, and artificially generated modifications. The various sample preparations resulted in complementary identifications, significantly increasing the depth of the analysis. Overall, 15,089 proteoforms from 2,780 protein accessions of human Caco-2 cells were identified, providing the most comprehensive dataset of this cell line up to now. The results from our study can serve as a guideline for designing and adapting TDP sample preparation strategies to particular research questions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-08 |
AnnouncementXML | Submission_2024-08-08_05:03:12.382.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Tholey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-02-20 11:14:34 | ID requested | |
⏵ 1 | 2024-08-08 05:03:12 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Top-Down Proteomics |
Proteoforms |
Sample Preparation |
Fractionation |
Mass spectrometry. |
Contact List
Andreas Tholey |
contact affiliation | Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, 24105 Kiel, Germany |
contact email | a.tholey@iem.uni-kiel.de |
lab head | |
Andreas Tholey |
contact affiliation | Systematic Proteome Research & Bioanalytics, University of Kiel |
contact email | a.tholey@iem.uni-kiel.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD049969
- Label: PRIDE project
- Name: Influence of Different Sample Preparation Approaches on Proteoform Identification by Top-Down Proteomics