PXD049450

PXD049450 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Establishment and characterization of fluorouracil and gemcitabine resistant cholangiocarcinoma cell lines
Description	Resistance to commonly used chemotherapeutic drugs is a major problem of cholangiocarcinoma (CCA) therapy, therefore more effective treatment strategy is urgently needed. Drug resistant cell lines have been used for elucidating drug resistance mechanisms in various cancers leading to the discovery of novel treatments. Here in, we established a 5-Fluorouracil and Gemcitabine resistant CCA cell lines. The resistant sublines were selected based on constant exposure of KKU-213B parental cells to 5-Fluorouracil and gemcitabine in a stepwise dose incremental strategy starting from below IC25 concentration). The cells that grown normally in the presence of 5-FU 7 and Gem 3 μM were used for subsequence experiments and weredesignated as KKU-213B-FR and KKU-213B-GR. The resultant cell lines were confirmed to be resistant to chemotherapeutic drug and also increased cell migration and invasion activity. In addition, the quantitative LC-MS/MS were utilized for the analysis of proteins involved in drug resistance mechanisms.
HostingRepository	jPOST
AnnounceDate	2025-02-18
AnnouncementXML	Submission_2025-02-17_07:00:06.568.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sittiruk Roytrakul
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-02-17 21:01:10	ID requested
⏵ 1	2025-02-17 07:00:06	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: CCA, Resistance

submitter keyword: CCA, Resistance

Contact List

Sumalee Obchoei
lab head
Sittiruk Roytrakul
contact affiliation	BIOTEC
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002940/