PXD049141
PXD049141 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | [HPP-2.0] Dynamic phosphotyrosine-dependent signaling profiling in living cells by two-dimensional proximity proteomics |
Description | Tyrosine phosphorylation (pTyr)-dependent signaling pathways play vital role in various biological processes, which are spatiotemporally assembled and dynamically regulated on minute-scale by pTyr in living cells. Studying these pTyr-mediated signaling complexes is therefore challenging due to the highly dynamic nature of the protein complexes and the low abundance of pTyr. In this study, we adopted minute-resolution APEX2-based proximity labeling (PL) in living cells and Src SH2 superbinder-based pTyr peptide enrichment for simultaneously profiling these protein complexes and associated pTyr sites from the same affinity-purified sample. Upon different time-course of EGF stimulation of the living cells stably expressing APEX2-FLAG-GRB2, we constructed two-dimensional time-course curves for both interactome and tyrosine phosphoproteome. |
HostingRepository | iProX |
AnnounceDate | 2024-02-03 |
AnnouncementXML | Submission_2024-02-03_19:46:35.807.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ruijun Tian |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-02-03 19:45:20 | ID requested | |
⏵ 1 | 2024-02-03 19:46:36 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: phosphotyrosine, living cells, proximity proteomics |
Contact List
Ruijun Tian | |
---|---|
contact affiliation | Southern University of Science and Technology |
contact email | tianrj@sustech.edu.cn |
lab head | |
Ruijun Tian | |
contact affiliation | Southern University of Science and Technology |
contact email | 13667356941@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |