PXD048601 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Extracellular vesicle formation in Cryptococcus deuterogattii impacts fungal virulence |
Description | Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity of benzimidazoles against Cryptococcus deuterogattii. In this study, during the phenotypic characterization of C. deuterogattii mutants expected to lack NOP16 expression, we observed a reduced EV production. Whole-genome sequencing, RNAseq, and cellular proteomics revealed that, contrary to our initial findings, these mutants expressed Nop16 but exhibited altered expression of 14 genes potentially involved in sugar transport. Based on this observation, we designated these mutants as past1 and past2, representing potentially altered sugar transport. Analysis of the small molecule composition of EVs produced by wild-type cells and the past1 and past2 mutants revealed not only a reduced number of EVs but also an altered small molecule composition. In a Galleria mellonella model of infection, the past1 and past2 mutants were hypovirulent. The hypovirulent phenotype was reverted when EVs produced by wild-type cells, but not mutant EVs, were co-injected with the mutant cells in G. mellonella. These results connect EV biogenesis, cargo and cryptococcal virulence. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-29 |
AnnouncementXML | Submission_2024-02-29_13:22:54.260.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marlon D M Santos |
SpeciesList | scientific name: Cryptococcus gattii VGII R265; NCBI TaxID: 294750; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-01-16 06:25:59 | ID requested | |
⏵ 1 | 2024-02-29 13:22:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: fungal virulence,Cryptococcus deuterogattii |
Contact List
Marcio L. Rodrigues |
contact affiliation | Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil. |
contact email | marciolrodrig@gmail.com |
lab head | |
Marlon D M Santos |
contact affiliation | Computational Mass Spectrometry & Proteomics Group - Fiocruz |
contact email | marlondms@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048601
- Label: PRIDE project
- Name: Extracellular vesicle formation in Cryptococcus deuterogattii impacts fungal virulence