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PXD048601

PXD048601 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtracellular vesicle formation in Cryptococcus deuterogattii impacts fungal virulence
DescriptionSmall molecules are components of fungal extracellular vesicles (EVs), but their biological roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity of benzimidazoles against Cryptococcus deuterogattii. In this study, during the phenotypic characterization of C. deuterogattii mutants expected to lack NOP16 expression, we observed a reduced EV production. Whole-genome sequencing, RNAseq, and cellular proteomics revealed that, contrary to our initial findings, these mutants expressed Nop16 but exhibited altered expression of 14 genes potentially involved in sugar transport. Based on this observation, we designated these mutants as past1 and past2, representing potentially altered sugar transport. Analysis of the small molecule composition of EVs produced by wild-type cells and the past1 and past2 mutants revealed not only a reduced number of EVs but also an altered small molecule composition. In a Galleria mellonella model of infection, the past1 and past2 mutants were hypovirulent. The hypovirulent phenotype was reverted when EVs produced by wild-type cells, but not mutant EVs, were co-injected with the mutant cells in G. mellonella. These results connect EV biogenesis, cargo and cryptococcal virulence.
HostingRepositoryPRIDE
AnnounceDate2024-02-29
AnnouncementXMLSubmission_2024-02-29_13:22:54.260.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarlon D M Santos
SpeciesList scientific name: Cryptococcus gattii VGII R265; NCBI TaxID: 294750;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-16 06:25:59ID requested
12024-02-29 13:22:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: fungal virulence,Cryptococcus deuterogattii
Contact List
Marcio L. Rodrigues
contact affiliationInstituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.
contact emailmarciolrodrig@gmail.com
lab head
Marlon D M Santos
contact affiliationComputational Mass Spectrometry & Proteomics Group - Fiocruz
contact emailmarlondms@gmail.com
dataset submitter
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Dataset FTP location
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